Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | solubilization in Triton X-100 results in higher activity compared to bovine serum albumin | Homo sapiens | |
phorbol 12-myristate 13-acetate | - |
Homo sapiens | |
TNF-alpha | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of FLAG-tagged isozyme in HEK293 cells | Homo sapiens |
expression of FLAG-tagged isozyme in HEK293 cells, expression of His-tagged isozyme in Sf9 insect cells via baculovirus infection system | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | substrate kinetic analysis | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NRA0 | isozyme Sk2; 2 isozymes SK1 and SK2 | - |
Homo sapiens | Q9NYA1 | isozyme Sk1; 2 isozymes SK1 and SK2 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.184 | - |
native isozyme SK1 in cell extract | Homo sapiens |
19.34 | - |
purified recombinant isozyme SK1 | Homo sapiens |
43.4 | - |
recombinant isozyme SK1 in cell extract of recombinant cells | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotinyl-D-erythro-sphingosine | - |
Homo sapiens | ADP + biotinyl-D-erythro-sphingosine 1-phosphate | - |
? | |
ATP + D-erythro-sphingosine | - |
Homo sapiens | ADP + D-erythro-sphingosine 1-phosphate | - |
? | |
additional information | isozyme SK1 shows high activity compared to isozyme SK2, development and optimization of a high-sensitive assay method utilizing biotinyl sphingosine and streptavidin-coated membranes with recombinant isozymes | Homo sapiens | ? | - |
? | |
additional information | isozyme SK2 shows low activity compared to isozyme SK1, development and optimization of a high-sensitive assay method utilizing biotinyl-sphingosine and streptavidin-coated membranes with recombinant isozymes | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SK | - |
Homo sapiens |
sphinganine kinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |