Literature summary for 2.7.1.74 extracted from

Smal, C.; Ntamashimikiro, S.; Arts, A.; Van Den Neste, E.; Bontemps, F.
Influence of phosphorylation of THR-3, SER-11, and SER-15 on deoxycytidine kinase activity and stability (2010), Nucleosides Nucleotides Nucleic Acids, 29, 404-407.

Search for more literature for 2.7.1.74

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, transient expression of His-tagged wild-type and mutants in HEK 293T cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
S11A	site-directed mutagenesis, replacement of the phosphorylation site	Homo sapiens
S11E	site-directed mutagenesis, replacement of the phosphorylation site	Homo sapiens
S15A	site-directed mutagenesis, replacement of the phosphorylation site	Homo sapiens
S15E	site-directed mutagenesis, replacement of the phosphorylation site, the mutant shows a slight, but significant, reduction of Ser74 phosphorylation	Homo sapiens
T3A	site-directed mutagenesis, replacement of the phosphorylation site, the mutant shows a slight, but significant, reduction of Ser74 phosphorylation	Homo sapiens
T3E	site-directed mutagenesis, replacement of the phosphorylation site, the mutant shows a slight, but significant, reduction of Ser74 phosphorylation	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	the enzyme contains four in vivo phosphorylation sites: Thr3, Ser11, Ser-15, and Ser74. Phosphorylation of Ser74, the major phosphorylated residue, strongly influences dCK activity in vivo. Phosphorylation of the three other sites, located in the N-terminal extremity of the protein, does not significantly modify enzyme activity, but phosphorylation of Thr3 can promote enzyme stability	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
lymphocyte	leukemic	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
dCK	-	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	deoxycytidine kinase is a key enzyme in the salvage of deoxyribonucleosides and in the activation of several anticancer and antiviral nucleoside analogues	Homo sapiens

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Release 2023.1 (January 2023)