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Literature summary for 2.7.1.74 extracted from

  • Hazra, S.; Konrad, M.; Lavie, A.
    The sugar ring of the nucleoside is required for productive substrate positioning in the active site of human deoxycytidine kinase (dCK): implications for the development of dCK-activated acyclic guanine analogues (2010), J. Med. Chem., 53, 5792-5800.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.74

Crystallization (Commentary)

Crystallization (Comment) Organism
dCK in complex with acyclovir at the nucleoside phosphoryl acceptor site, and UDP at the phosphoryl donor site, hanging drop vapour diffusion method, 20 mg/ml protein with 5 mM UDP and 5 mM acyclovir is mixed with reservoir solution containing 0.90-1.5 M trisodium citrate dihydrate and 100 mM HEPES, pH 7.5, 12°C, X-ray diffraction structure determination and analysis at 2.1 A resolution, molecular replacement Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + deoxyadenosine Homo sapiens
-
 ADP + dAMP
-
 ?
ATP + deoxycytidine Homo sapiens
-
 ADP + dCMP
-
 ?
ATP + deoxyguanosine Homo sapiens
-
 ADP + dGMP
-
 ?
UTP + deoxyadenosine Homo sapiens
-
 UDP + dAMP
-
 ?
UTP + deoxycytidine Homo sapiens
-
 UDP + dCMP
-
 ?
UTP + deoxyguanosine Homo sapiens
-
 UDP + dGMP
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P27707
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deoxyadenosine
-
 Homo sapiens ADP + dAMP
-
 ?
ATP + deoxycytidine
-
 Homo sapiens ADP + dCMP
-
 ?
ATP + deoxyguanosine
-
 Homo sapiens ADP + dGMP
-
 ?
additional information the human enzyme is not active with the acyclic guanine analogue acyclovir. Acyclovir binds at the dCK active site, but does so adopting a nonproductive conformation. Despite binding ACV, the enzyme remains in the open, inactive state, overview. dCK would phosphorylate acyclic guanine analogs if they can induce a similar rotation Homo sapiens ?
-
 ?
UTP + deoxyadenosine
-
 Homo sapiens UDP + dAMP
-
 ?
UTP + deoxycytidine
-
 Homo sapiens UDP + dCMP
-
 ?
UTP + deoxyguanosine
-
 Homo sapiens UDP + dGMP
-
 ?

Synonyms

Synonyms Comment Organism
dCK
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens
UTP
-
 Homo sapiens