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Literature summary for 2.7.1.74 extracted from

  • Zhang, Y.; Secrist, J.A.; Ealick, S.E.
    The structure of human deoxycytidine kinase in complex with clofarabine reveals key interactions for prodrug activation (2006), Acta Crystallogr. Sect. D, 62, 133-139.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His-tag fusion protein in Escherichia coli BL21(DE3) Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, in complex with 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine and ADP Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NTP + 2'-deoxyadenosine Homo sapiens
-
NDP + 2'-deoxyadenosine 5'-phosphate
-
?
NTP + 2'-deoxycytidine Homo sapiens more effective substrate than 2'-deoxyadenosine and 2'-deoxyguanosine NDP + 2'-deoxycytidine 5'-phosphate
-
?
NTP + 2'-deoxyguanosine Homo sapiens
-
NDP + 2'-deoxyguanosine 5'-phosphate
-
?
NTP + 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine Homo sapiens i.e. clofarabine, rate-limiting step in the activation of the prodrug, precursor of an effective chemotherapeutic agent for leukemias and other haematological malignancies NDP + 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine 5'-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P27707
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NTP + 2'-deoxyadenosine
-
Homo sapiens NDP + 2'-deoxyadenosine 5'-phosphate
-
?
NTP + 2'-deoxycytidine more effective substrate than 2'-deoxyadenosine and 2'-deoxyguanosine Homo sapiens NDP + 2'-deoxycytidine 5'-phosphate
-
?
NTP + 2'-deoxyguanosine
-
Homo sapiens NDP + 2'-deoxyguanosine 5'-phosphate
-
?
NTP + 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine i.e. clofarabine, rate-limiting step in the activation of the prodrug, precursor of an effective chemotherapeutic agent for leukemias and other haematological malignancies Homo sapiens NDP + 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine 5'-phosphate
-
?

Subunits

Subunits Comment Organism
dimer crystal structure analysis Homo sapiens

Synonyms

Synonyms Comment Organism
dCK
-
Homo sapiens