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Literature summary for 2.7.1.74 extracted from

  • Someya, H.; Shaddix, S.C.; Tiwari, K.N.; Secrist, J.A., 3rd; Parker, W.B.
    Phosphorylation of 4'-thio-beta-D-arabinofuranosylcytosine and its analogs by human deoxycytidine kinase (2003), J. Pharmacol. Exp. Ther., 304, 1314-1322.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4'-thio-beta-D-arabinofuranosylcytosine IC50 0.000024 mM Homo sapiens
5-chloro-4'-thio-beta-D-arabinofuranosylcytosine IC50 0.051 mM Homo sapiens
5-fluoro-4'-thio-beta-D-arabinofuranosylcytosine IC50 0.0000020 mM Homo sapiens
cytosine arabinoside IC50 0.0000055 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00013
-
deoxycytidine cosubstrate UTP 37°C, pH 8.0 Homo sapiens
0.00032
-
4'-thio-2'-deoxycytidine cosubstrate ATP 37°C, pH 8.0 Homo sapiens
0.00041
-
beta-D-arabinofuranosylcytosine cosubstrate UTP 37°C, pH 8.0 Homo sapiens
0.00057
-
deoxycytidine cosubstrate ATP 37°C, pH 8.0 Homo sapiens
0.015
-
beta-D-arabinofuranosylcytosine cosubstrate ATP 37°C, pH 8.0 Homo sapiens
0.02
-
4'-thio-beta-D-arabinofuranosylcytosine cosubstrate UTP 37°C, pH 8.0 Homo sapiens
0.088
-
4'-thio-beta-D-arabinofuranosylcytosine cosubstrate ATP 37°C, pH 8.0 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UTP + deoxycytidine Homo sapiens UTP is physiological phosphate donor UDP + dCMP
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4'-thio-2'-deoxycytidine + UTP
-
Homo sapiens UDP + 4'-thio-2'-deoxycytidine 5'-phosphate
-
?
4'-thio-beta-D-arabinofuranosylcytosine + UTP
-
Homo sapiens UDP + 4'-thio-beta-D-arabinofuranosylcytosine 5'-phosphate
-
?
NTP + cytosine arabinoside
-
Homo sapiens NDP + cytosine arabinoside 5'-monophosphate
-
?
NTP + deoxycytidine
-
Homo sapiens NDP + dCMP
-
?
UTP + deoxycytidine UTP is physiological phosphate donor Homo sapiens UDP + dCMP
-
?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000002
-
IC50 0.0000020 mM Homo sapiens 5-fluoro-4'-thio-beta-D-arabinofuranosylcytosine
0.0000055
-
IC50 0.0000055 mM Homo sapiens cytosine arabinoside
0.000024
-
IC50 0.000024 mM Homo sapiens 4'-thio-beta-D-arabinofuranosylcytosine
0.051
-
IC50 0.051 mM Homo sapiens 5-chloro-4'-thio-beta-D-arabinofuranosylcytosine