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Literature summary for 2.7.1.74 extracted from
- Kinetic properties and inhibition of human T lymphoblast deoxycytidine kinase (1989), J. Biol. Chem., 264, 9359-9364.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | kinetics | Homo sapiens |  |
| CMP | - |
Homo sapiens | |
| CTP | - |
Homo sapiens | |
| Cytosine arabinoside monophosphate | strong | Homo sapiens | |
| Cytosine arabinoside triphosphate | - |
Homo sapiens | |
| dAMP | strong | Homo sapiens | |
| dATP | strong | Homo sapiens | |
| dCDP | - |
Homo sapiens | |
| dCMP | product inhibition, strong | Homo sapiens | |
| dCTP | kinetics | Homo sapiens | |
| dGMP | strong | Homo sapiens | |
| Dideoxyadenosine | - |
Homo sapiens | |
| Dideoxycytidine | - |
Homo sapiens | |
| dUMP | strong | Homo sapiens | |
| dUTP | - |
Homo sapiens | |
| GDP | - |
Homo sapiens | |
| IMP | - |
Homo sapiens | |
| P1,P4-diadenosine 5'-tetraphosphate | not di-, tri- or pentaphosphate derivative | Homo sapiens | |
| Sodium diphosphate | not monophosphate | Homo sapiens | |
| UDP | strong | Homo sapiens | |
| UMP | strong | Homo sapiens | |
| UTP | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens | |
| 0.0008 | 0.0015 | deoxycytidine | 37°C, pH 7.0 | Homo sapiens | |
| 0.02 | 0.04 | cytosine arabinoside | pH 7.0, 37°C | Homo sapiens | |
| 0.33 | 0.43 | deoxyguanosine | 37°C, pH 7.0 | Homo sapiens | |
| 0.45 | - |
cytidine | 37°C, pH 7.0 | Homo sapiens | |
| 0.5 | - |
deoxyadenosine | 37°C, pH 7.0 | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mg2+/ATP complex is the reactive species | Homo sapiens | |
| Mg2+ | maximal activity at a ATP:Mg2+-ratio of 1:1 | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leukemia cell | leukemic T-lymphoblasts | Homo sapiens | - |
| leukemia cell | cell line MOLT-4 | Homo sapiens | - |
| MOLT-4 cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8 | - |
37°C, pH 7.0 | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2'-deoxycytidine | - |
Homo sapiens | ADP + 5'-dCMP | - |
? | |
| ATP + cytidine | - |
Homo sapiens | ADP + ? | - |
? | |
| NTP + cytosine arabinoside | - |
Homo sapiens | NDP + cytosine arabinoside 5'-monophosphate | - |
? | |
| NTP + deoxyadenosine | - |
Homo sapiens | NDP + dAMP | - |
? | |
| NTP + deoxycytidine | enzyme shows little specificity towards phosphate donor | Homo sapiens | NDP + dCMP | - |
? | |
| NTP + deoxyguanosine | - |
Homo sapiens | NDP + dGMP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
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