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Literature summary for 2.7.1.74 extracted from

  • Durham, J.P.; Ives, D.H.
    Deoxycytidine kinase. II. Purification and general properties of the calf thymus enzyme (1970), J. Biol. Chem., 245, 2276-2284.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activation Bos taurus
dithiothreitol activation Bos taurus

General Stability

General Stability Organism
2-mercaptoethanol stabilizes Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
ADP not Bos taurus
CMP not Bos taurus
cytidine cytosine arabinoside as substrate Bos taurus
cytosine arabinoside deoxyguanosine as substrate Bos taurus
dAMP not Bos taurus
dCDP
-
Bos taurus
dCMP
-
Bos taurus
dCTP reversible by dTTP; reversible by UTP, dUTP Bos taurus
deoxyadenosine cytosine arabinoside or deoxyguanosine as substrate Bos taurus
deoxycytidine cytosine arabinoside as substrate; deoxyadenosine as substrate; deoxyguanosine as substrate Bos taurus
deoxyguanosine cytosine arabinoside as substrate Bos taurus
deoxythymidine cytosolic isozyme I, not Bos taurus
dTDP
-
Bos taurus
dUMP not Bos taurus
GDP not Bos taurus
GSSG thiol reagents protect or reverse Bos taurus
HgCl2 strong, thiol reagents protect Bos taurus
additional information little or no inhibition by AMP, dTMP, adenosine, guanosine; little or no inhibition by deoxyuridine Bos taurus
N-ethylmaleimide thiol reagents protect or reverse Bos taurus
p-chloromercuribenzoate thiol reagents protect or reverse Bos taurus
UDP
-
Bos taurus
UMP not Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Bos taurus
Ca2+ about 55% as efficient as Mg2+ Bos taurus
CdCl2 activation, in the absence of dithiothreitol Bos taurus
Co2+ activation Bos taurus
Co2+ about 40% as efficient as Mg2+ Bos taurus
Fe2+ activation Bos taurus
Fe2+ about 45% as efficient as Mg2+ Bos taurus
Mg2+
-
Bos taurus
Mn2+ requirement Bos taurus
Mn2+ about 80% as efficient as Mg2+ Bos taurus
additional information no activation by Ba2+, Cu2+ Bos taurus
Ni2+ activation Bos taurus
Ni2+ about 20% as efficient as Mg2+ Bos taurus
Sr2+ activation Bos taurus
Sr2+ about 15% as efficient as Mg2+ Bos taurus
Zn2+ activation Bos taurus
Zn2+ about 40% as efficient as Mg2+ Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
gel filtration, equilibrium density and sucrose density gradient centrifugation Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.012
-
37°C, pH 8.0 Bos taurus

Storage Stability

Storage Stability Organism
-30°C, several months in the presence of 2-mercaptoethanol Bos taurus
4°C, if purified in the absence of 2-mercaptoethanol it loses most of its activity within 2 days, DTT restores Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxycytidine in the presence of DTT less effective than dUTP, UTP, dTTP or GTP, without DTT only less effective than dUTP Bos taurus ADP + 5'-dCMP
-
?
dTTP + 2'-deoxycytidine
-
Bos taurus dTDP + 2'-deoxy-CMP
-
?
dUTP + 2'-deoxycytidine best phosphate donor Bos taurus dUDP + 2'-deoxy-CMP
-
?
GTP + 2'-deoxycytidine
-
Bos taurus GDP + 2'-deoxy-CMP
-
?
NTP + cytosine arabinoside
-
Bos taurus NDP + cytosine arabinoside 5'-monophosphate
-
?
NTP + deoxyadenosine
-
Bos taurus NDP + dAMP
-
?
NTP + deoxycytidine
-
Bos taurus NDP + dCMP
-
?
NTP + deoxyguanosine
-
Bos taurus NDP + dGMP
-
?
UTP + 2'-deoxycytidine
-
Bos taurus UDP + 2'-deoxy-CMP
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
at least 24 h, in the presence of dithiothreitol Bos taurus
60
-
dCTP enhances heat stability at pH 6, dithiothreitol slightly, not deoxycytidine or other nucleosides Bos taurus
60
-
t1/2: 7.9 min at pH 8, t1/2: 2 min at pH 6, unstable at pH 10 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 10 broad plateau, with very slight optima at pH 6 and 10 Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
t1/2: 2 min at 60°C Bos taurus
8
-
moderately stable, t1/2: 7.9 min at 60°C Bos taurus
10
-
unstable at 60°C Bos taurus