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Literature summary for 2.7.1.68 extracted from

  • Kusano, H.; Testerink, C.; Vermeer, J.E.; Tsuge, T.; Shimada, H.; Oka, A.; Munnik, T.; Aoyama, T.
    The arabidopsis phosphatidylinositol phosphate 5-kinase PIP5K3 is a key regulator of root hair tip growth (2008), Plant Cell, 20, 367-380.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.68

Cloned(Commentary)

Cloned (Comment) Organism
a yellow fluorescent (YFP) und GUS fusion PIP5K3 protein is expressed in Escherichia coli cells and transformed in Arabidopsis thaliana to produce trangenic plants. Overexpression and mutant is investigated. Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information cytoplasmic space of elongating root hair apices Arabidopsis thaliana
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plasma membrane
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 Arabidopsis thaliana 5886
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Metals/Ions

Metals/Ions Comment Organism Structure
K+ 70 mM in the activity assay Arabidopsis thaliana
Mg2+ 10 mM in the activity assay Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate Arabidopsis thaliana
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 ADP + 1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate PIP5K3 is involved in localizing 1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate to the elongating root hair apex and is a key regulator of the machinery that initiates and promotes root hair tip growth. r
additional information Arabidopsis thaliana GST-PIP5K3 produces 1-phosphatidyl-1D-myo-inositol 4,5 by phosphorylating 1-phosphatidyl-1D-myo-inositol 4-phosphate, both the natural and synthetic forms. Its also phosphorylates 1-phosphatidyl-1D-myo-inositol 5-phosphate, but less efficiently. PIP5K3 catalyzes the synthesis of 1-phosphatidyl-1D-myo-inositol 4,5 preferentially from 1-phosphatidyl-1D-myo-inositol 4-phosphate in vitro. ?
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 ?
additional information Arabidopsis thaliana PIP5K3 may function as a downstream effector of rho-related GTPases of plants (ROPs) in regulatory mechanisms, not only for the planar polarity of bulge initiation but also for the promotion of root hair tip growth. ?
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 ?
additional information Arabidopsis thaliana Reduced expression of functional PIP5K3 is responsible for the short-root hair phenotype. Inducible overexpression of PIP5K3 enhanced root hair elongation and increased the rate of 1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate ?
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 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O48709 Used Arabidopsis thaliana lines are in the Columbia background, and Columbia is used as the wild type.
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Purification (Commentary)

Purification (Comment) Organism
Partial purification from Escherichia coli culture cells. The cells are suspended in PBS buffer, sonicated on ice, and centrifuged. Supernatant is mixed with glutathione-Sepharose 4B and incubated at 48°C for 2 h. The Sepharose beads are collected and washed with an excess volume of PBS. Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
cell culture Escherichia coli BL21 cell is used for cloning and expression Arabidopsis thaliana
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root hair t-DNA insertion mutations that reduces the expression of PIP5K3 cause shorter root hairs than in the wild type. Overexpression causes longer root hairs and multiple protruding sites on a single trichoblast. Arabidopsis thaliana
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
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 Arabidopsis thaliana ADP + 1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate PIP5K3 is involved in localizing 1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate to the elongating root hair apex and is a key regulator of the machinery that initiates and promotes root hair tip growth. r
additional information GST-PIP5K3 produces 1-phosphatidyl-1D-myo-inositol 4,5 by phosphorylating 1-phosphatidyl-1D-myo-inositol 4-phosphate, both the natural and synthetic forms. Its also phosphorylates 1-phosphatidyl-1D-myo-inositol 5-phosphate, but less efficiently. PIP5K3 catalyzes the synthesis of 1-phosphatidyl-1D-myo-inositol 4,5 preferentially from 1-phosphatidyl-1D-myo-inositol 4-phosphate in vitro. Arabidopsis thaliana ?
-
 ?
additional information PIP5K3 may function as a downstream effector of rho-related GTPases of plants (ROPs) in regulatory mechanisms, not only for the planar polarity of bulge initiation but also for the promotion of root hair tip growth. Arabidopsis thaliana ?
-
 ?
additional information Reduced expression of functional PIP5K3 is responsible for the short-root hair phenotype. Inducible overexpression of PIP5K3 enhanced root hair elongation and increased the rate of 1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate Arabidopsis thaliana ?
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 ?

Synonyms

Synonyms Comment Organism
phosphatidylinositol 4-phosphate 5-kinase
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 Arabidopsis thaliana
phosphatidylinositol phosphate 5-kinase
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 Arabidopsis thaliana
PIP5K Arabidopsis genome contains 11 genes encoding proteins with significant sequence similarity to animal PIP5Ks. These are classified into two subfamilies: subfamily A proteins (PIP5K10 and PIP5K11) consist of dimerization and catalytic domains similar to those in the animal enzymes, while subfamily B proteins (PIP5K1 to PIP5K9) have an additional domain containing a repeat of the membrane occupation and recognition nexus motif at the N-terminus. Arabidopsis thaliana
PIP5K3 PIP5K3 encodes the enzyme activity producing PtdIns(4,5)P2. Arabidopsis thaliana
type I PtdInsP kinase
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 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
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 asay at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
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 Arabidopsis thaliana