Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | above 20 mM PI kinase I and II are inhibited. PI kinase III is only slightly inhibited at 50 mM | Oryctolagus cuniculus | |
NaCl | above 0.1 mM | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Oryctolagus cuniculus | |
0.1 | - |
ATP | PI kinase I | Oryctolagus cuniculus | |
0.1 | 0.15 | ATP | PI kinase III | Oryctolagus cuniculus | |
0.2 | - |
ATP | PI kinase II | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the three enzyme forms are completely dependent on Mg2+, optimal activity at 4-10 mM | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
PI kinase I, II and III | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
reticulocyte | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol | - |
Oryctolagus cuniculus | ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | PI kinase III | Oryctolagus cuniculus |
7.5 | 9 | PI kinase I | Oryctolagus cuniculus |
9 | - |
PI kinase II | Oryctolagus cuniculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | pH 7.5: about 75% of maximal activity, pH 10.5: about 40% of maximal activity, PI kinase III | Oryctolagus cuniculus |
7.5 | 10.5 | pH 7.5: about 50% of maximal activity, pH 10.5: about 65% of maximal activity, PI kinase I | Oryctolagus cuniculus |