Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose-1,6-bisphosphate | allosteric effector, binds to PykF but not to PykA | Yersinia enterocolitica |
Cloned (Comment) | Organism |
---|---|
recombinant expression of isozymes PykA and PykF in Escherichia coli. Solubility of both PykA and PykF is superior when expression cultures are incubated at 27°C compared to 37°C, so that for large-scale production both proteins are expressed at 27°C after induction for 5 h | Yersinia enterocolitica |
Crystallization (Comment) | Organism |
---|---|
purified recombinant untagged enzyme, synchrotron radiation small-angle X-ray scattering structure determination and analysis | Yersinia enterocolitica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Yersinia enterocolitica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50500 | - |
4 * 50500, PykF, SDS-PAGE, circular dichroism spectroscopy, and gel filtration, 4 * 51500, PykA, SDS-PAGE, circular dichroism spectroscopy, and gel filtration | Yersinia enterocolitica |
51500 | - |
4 * 50500, PykF, SDS-PAGE, circular dichroism spectroscopy, and gel filtration, 4 * 51500, PykA, SDS-PAGE, circular dichroism spectroscopy, and gel filtration | Yersinia enterocolitica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate | Yersinia enterocolitica | - |
ADP + phosphoenolpyruvate | - |
? | |
ATP + pyruvate | Yersinia enterocolitica WA-314 | - |
ADP + phosphoenolpyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia enterocolitica | - |
isozymes PykA and PykF encoded by genes pykA and pykF | - |
Yersinia enterocolitica WA-314 | - |
isozymes PykA and PykF encoded by genes pykA and pykF | - |
Purification (Comment) | Organism |
---|---|
recombinant untagged isozymes PykA and PykF 2.71fold and 2.1fold, respectively, from Escherichia coli to near homogeneity by anion exchange chromatography and gel filtration | Yersinia enterocolitica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
85 | - |
purified recombinant PykA, pH 7.5, 30°C | Yersinia enterocolitica |
108 | - |
purified recombinant PykF, pH 7.5, 30°C | Yersinia enterocolitica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate | - |
Yersinia enterocolitica | ADP + phosphoenolpyruvate | - |
? | |
ATP + pyruvate | - |
Yersinia enterocolitica WA-314 | ADP + phosphoenolpyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 50500, PykF, SDS-PAGE, circular dichroism spectroscopy, and gel filtration, 4 * 51500, PykA, SDS-PAGE, circular dichroism spectroscopy, and gel filtration | Yersinia enterocolitica |
Synonyms | Comment | Organism |
---|---|---|
PykA | - |
Yersinia enterocolitica |
PykF | - |
Yersinia enterocolitica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Yersinia enterocolitica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Yersinia enterocolitica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Yersinia enterocolitica |