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Literature summary for 2.7.1.40 extracted from

  • Sandoval, W.; Isea, R.; Rodriguez, E.; Ramirez, J.L.
    A biochemical and genetic study of Leishmania donovani pyruvate kinase (2008), Gene, 424, 25-32.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 2,6-bisphosphate allosteric stimulation Leishmania donovani
additional information no stimulation: fructose 1,6-bisphosphate Leishmania donovani

Cloned(Commentary)

Cloned (Comment) Organism
-
Leishmania donovani

Protein Variants

Protein Variants Comment Organism
H480Q site-directed mutagenesis of putative binding site of fructose 2,6-bisphosphate. Mutant displays hyperbolic kinetics that is not changed by addition of the allosteric effector fructose 2,6-bisphosphate Leishmania donovani
K453L site-directed mutagenesis of putative binding site of fructose 2,6-bisphosphate. Mutant retains a sigmoidal kinetics and is little affected by addition of fructose 2,6-bisphosphate Leishmania donovani
K453L/H480Q site-directed mutagenesis of putative binding sites of fructose 2,6-bisphosphate. Mutant displays hyperbolic kinetics Leishmania donovani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information in the presence of the effector fructose 2,6-bisphosphate the enzyme displays a hyperbolic saturation curve, whereas in the absence of fructose 2,6-bisphosphate the curve is sigmoid. The phosphoenolpyruvate concentration for S0.5 in presence of fructose 2,6-bisphosphate is 0.08 mM, and 1.16 mM in absence, with a concomitant increase in Vmax from 492.1 U/mg in absence to 506.99 U/mg in presence of fructose 2,6-bisphosphate. For ADP, a Km of 0.32 mM has been calculated Leishmania donovani

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54400
-
x * 54400, calculated Leishmania donovani

Organism

Organism UniProt Comment Textmining
Leishmania donovani A7LIU4
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
68.2
-
mutant H480Q, pH 7.2, 25°C Leishmania donovani
98
-
mutant K453L/H480Q, pH 7.2, 25°C Leishmania donovani
156.9
-
mutant K453L, pH 7.2, 25°C Leishmania donovani
178.1
-
wild-type, pH 7.2, 25°C Leishmania donovani

Subunits

Subunits Comment Organism
? x * 54400, calculated Leishmania donovani

pI Value

Organism Comment pI Value Maximum pI Value
Leishmania donovani calulated
-
6.5