Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not activated by fructose 1,6-bisphosphate | Sus scrofa | |
Triton X-100 | 50% increase of activity of the bound enzyme only | Sus scrofa |
General Stability | Organism |
---|---|
the enzyme cannot be solubilized in detergents, but trypsin without loss of activity | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by L-alanine | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | 0.05 | phosphoenolpyruvate | bound enzyme | Sus scrofa | |
0.02 | 0.03 | phosphoenolpyruvate | solubilized enzyme | Sus scrofa | |
0.46 | - |
ADP | solubilized enzyme | Sus scrofa | |
0.52 | 0.56 | ADP | bound enzyme | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64500 | - |
SDS-PAGE, subunit | Sus scrofa |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | cannot be solubilized in detergents without loss of activity | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Fractogel EMD SO3- ion exchange chromatography and Fractogel EMD propyl (S) hydrophobic interaction chromatography | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood | - |
Sus scrofa | - |
erythrocyte | - |
Sus scrofa | - |
kidney | - |
Sus scrofa | - |
spermatozoon | in the acrosome of the sperm head and in the fibrous sheath of the principal piece of the flagellum | Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.12 | - |
sperm homogenate | Sus scrofa |
116.7 | - |
after 972fold purification | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | - |
Sus scrofa | ATP + pyruvate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PK-S | - |
Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Sus scrofa |