Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E117K | decreased activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP-Cr2+ | - |
Homo sapiens | |
oxalate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
phosphoenolpyruvate | wild type enzyme, in the presence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C | Homo sapiens | |
0.24 | - |
phosphoenolpyruvate | mutant enzyme E117K, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C | Homo sapiens | |
0.61 | - |
phosphoenolpyruvate | wild type enzyme, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C | Homo sapiens | |
2 | - |
ADP | mutant enzyme E117K, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C | Homo sapiens | |
2.3 | - |
ADP | wild type enzyme, in the presence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C | Homo sapiens | |
14.1 | - |
ADP | wild type enzyme, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°Cin 50 mM Mes-Tris, pH 6.0, at 25°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | essential activator, in the presence of K+ the affinities for phosphoenolpyruvate, ADP, ADP-Cr2+, and oxalate are 2-6fold higher than in the absence of K+ when ADP cannot bind to the enzyme until phosphoenolpyruvate forms a competent active site | Homo sapiens | |
Mg | required for activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE column chromatography and carboxymethyl-Sepharose column chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | - |
in the absence of K+ | Homo sapiens |
250 | - |
in the presence of K+ | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | - |
Homo sapiens | ATP + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | x-ray crystallography | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
phosphoenolpyruvate kinase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.2 | - |
phosphoenolpyruvate | wild type enzyme, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C | Homo sapiens | |
161 | - |
phosphoenolpyruvate | mutant enzyme E117K, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C | Homo sapiens | |
1182 | - |
phosphoenolpyruvate | wild type enzyme, in the presence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
ADP-Cr2+ | mutant enzyme E117K, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C | Homo sapiens | |
0.0013 | - |
ADP-Cr2+ | wild type enzyme, in the presence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C | Homo sapiens | |
0.0028 | - |
ADP-Cr2+ | wild type enzyme, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C | Homo sapiens | |
0.0048 | - |
oxalate | mutant enzyme E117K, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C | Homo sapiens | |
0.029 | - |
oxalate | wild type enzyme, in the presence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C | Homo sapiens | |
0.077 | - |
oxalate | wild type enzyme, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C | Homo sapiens |