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Literature summary for 2.7.1.40 extracted from

  • Oria-Hernandez, J.; Cabrera, N.; Perez-Montfort, R.; Ramirez-Silva, L.
    Pyruvate kinase revisited: the activating effect of K+ (2005), J. Biol. Chem., 280, 37924-37929.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
E117K decreased activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ADP-Cr2+
-
Homo sapiens
oxalate
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
phosphoenolpyruvate wild type enzyme, in the presence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
0.24
-
phosphoenolpyruvate mutant enzyme E117K, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
0.61
-
phosphoenolpyruvate wild type enzyme, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
2
-
ADP mutant enzyme E117K, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
2.3
-
ADP wild type enzyme, in the presence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
14.1
-
ADP wild type enzyme, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°Cin 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
K+ essential activator, in the presence of K+ the affinities for phosphoenolpyruvate, ADP, ADP-Cr2+, and oxalate are 2-6fold higher than in the absence of K+ when ADP cannot bind to the enzyme until phosphoenolpyruvate forms a competent active site Homo sapiens
Mg required for activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE column chromatography and carboxymethyl-Sepharose column chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
in the absence of K+ Homo sapiens
250
-
in the presence of K+ Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
Homo sapiens ATP + pyruvate
-
?

Subunits

Subunits Comment Organism
tetramer x-ray crystallography Homo sapiens

Synonyms

Synonyms Comment Organism
phosphoenolpyruvate kinase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2
-
phosphoenolpyruvate wild type enzyme, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
161
-
phosphoenolpyruvate mutant enzyme E117K, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
1182
-
phosphoenolpyruvate wild type enzyme, in the presence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ADP
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0005
-
ADP-Cr2+ mutant enzyme E117K, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
0.0013
-
ADP-Cr2+ wild type enzyme, in the presence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
0.0028
-
ADP-Cr2+ wild type enzyme, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
0.0048
-
oxalate mutant enzyme E117K, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
0.029
-
oxalate wild type enzyme, in the presence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens
0.077
-
oxalate wild type enzyme, in the absence of K+, in 50 mM Mes-Tris, pH 6.0, at 25°C Homo sapiens