Protein Variants | Comment | Organism |
---|---|---|
S22A | mutant protein activity is decreased by as much as 90% when compared with wild-type, is more active in the absence of fructose 1,6-bisphosphate | Saccharomyces cerevisiae |
T94A | activity similar to the wild type enzyme | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae JT20454 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sephadex G25 gel filtration, DEAE-cellulose column chromatography, phosphocellulose column chromatography, and ammonium sulfate precipitation | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | - |
Saccharomyces cerevisiae | ATP + pyruvate | - |
? | |
ADP + phosphoenolpyruvate | - |
Saccharomyces cerevisiae JT20454 | ATP + pyruvate | - |
? | |
additional information | pyruvate kinase is a substrate of protein kinase A | Saccharomyces cerevisiae | ? | - |
? | |
additional information | pyruvate kinase is a substrate of protein kinase A | Saccharomyces cerevisiae JT20454 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Pyk1 | - |
Saccharomyces cerevisiae |
pyruvate kinase 1 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Saccharomyces cerevisiae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharomyces cerevisiae | 2D-PAGE | - |
7.5 |