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Literature summary for 2.7.1.40 extracted from

  • Knowles, V.L.; Smith, C.S.; Smith, C.R.; Plaxton, W.C.
    Structural and regulatory properties of pyruvate kinase from the cyanobacterium Synechococcus PCC 6301 (2001), J. Biol. Chem., 276, 20966-20972.
    View publication on PubMed
Search for more literature for 2.7.1.40

Activating Compound

Activating Compound Comment Organism Structure
AMP at 1 mM or below Synechococcus sp.
D-ribose 5-phosphate
-
 Synechococcus sp.
glycerol 3-phosphate
-
 Synechococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate at 5 mM, pH 7.0, 35% inhibition Synechococcus sp.
ATP at 1 mM, pH 7.0, 50% inhibition Synechococcus sp.
citrate at 5 mM, pH 7.0, 40% inhibition Synechococcus sp.
fructose 1,6-diphosphate at 5 mM, pH 7.0, 60% inhibition Synechococcus sp.
phosphate at 5 mM, pH 7.0, 40% inhibition Synechococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
 phosphoenolpyruvate allosteric enzyme, pH 7.5, 24°C Synechococcus sp.
0.54
-
 phosphoenolpyruvate allosteric enzyme, pH 7.0, 24°C Synechococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
K+ not Synechococcus sp.
Mg2+ requirement Synechococcus sp.
Mg2+ either Mn2+ or Mg2+ are required Synechococcus sp.
Mn2+ either Mn2+ or Mg2+ are required Synechococcus sp.
Na+ not Synechococcus sp.
NH4+ not Synechococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 4 * 66000, SDS-PAGE Synechococcus sp.
280000
-
 gel filtration Synechococcus sp.

Organism

Organism UniProt Comment Textmining
Synechococcus sp.
-
 cyanobacterium, strain PCC 6301
-

Purification (Commentary)

Purification (Comment) Organism
-
 Synechococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate ADP preferred substrate, UDP, IDP, GDP and CDP may also be used with lower effectivity Synechococcus sp. ATP + pyruvate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 66000, SDS-PAGE Synechococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Synechococcus sp.