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Literature summary for 2.7.1.40 extracted from
- Molecular characterization, enzyme properties and transcriptional regulation of phosphoenolpyruvate carboxykinase and pyruvate kinase in a ruminal bacterium, Selenomonas ruminantium (2001), Microbiology, 147, 681-690.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| aspartate | - |
Selenomonas ruminantium |  |
| D-fructose 1,6-diphosphate | - |
Selenomonas ruminantium | |
| phosphoenolpyruvate | - |
Selenomonas ruminantium | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | severe, restored by addition of fructose 1,6-diphosphate | Selenomonas ruminantium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
phosphoenolpyruvate | allosteric enzyme, pH 7.0 | Selenomonas ruminantium | |
| 0.24 | - |
ADP | allosteric enzyme, pH 7.0 | Selenomonas ruminantium | |
| 0.35 | - |
ATP | allosteric enzyme, pH 7.0 | Selenomonas ruminantium | |
| 0.48 | - |
pyruvate | allosteric enzyme, pH 7.0 | Selenomonas ruminantium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | activates | Selenomonas ruminantium | |
| Mg2+ | either Mn2+ or Mg2+ are required | Selenomonas ruminantium | |
| Mn2+ | either Mn2+ or Mg2+ are required | Selenomonas ruminantium | |
| Na+ | not | Selenomonas ruminantium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51300 | - |
4 * 51300, SDS-PAGE | Selenomonas ruminantium |
| 200000 | - |
gel filtration | Selenomonas ruminantium |
| 250000 | - |
gel filtration | Selenomonas ruminantium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Selenomonas ruminantium | Q9LBS6 | ruminal bacterium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Selenomonas ruminantium |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate | - |
Selenomonas ruminantium | ATP + pyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 51300, SDS-PAGE | Selenomonas ruminantium |
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