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Literature summary for 2.7.1.40 extracted from
- Purification and characterization of cytosolic pyruvate kinase from developing seeds of Brassica campestris L (2000), Indian J. Biochem. Biophys., 37, 51-58.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 1,6-diphosphate | - |
Brassica rapa subsp. oleifera |  |
| D-glucose 6-phosphate | - |
Brassica rapa subsp. oleifera | |
| phosphate | - |
Brassica rapa subsp. oleifera | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| citrate | - |
Brassica rapa subsp. oleifera | |
| fumarate | - |
Brassica rapa subsp. oleifera | |
| glutamine | - |
Brassica rapa subsp. oleifera | |
| isocitrate | - |
Brassica rapa subsp. oleifera | |
| L-glutamate | - |
Brassica rapa subsp. oleifera | |
| oxalate | - |
Brassica rapa subsp. oleifera | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
phosphoenolpyruvate | pH 6.8, 30°C | Brassica rapa subsp. oleifera | |
| 0.11 | - |
ADP | pH 6.8, 30°C | Brassica rapa subsp. oleifera | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Brassica rapa subsp. oleifera | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | fulfills requirement for monovalent cation | Brassica rapa subsp. oleifera | |
| Mg2+ | fulfills absolute requirement for divalent cation | Brassica rapa subsp. oleifera | |
| additional information | absolute requirement for a bivalent and a monovalent cation with Mg2+ and K+ fulfilling this | Brassica rapa subsp. oleifera |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000 + 4-x * 57000, SDS-PAGE | Brassica rapa subsp. oleifera |
| 57000 | - |
x * 55000 + 4-x * 57000, SDS-PAGE | Brassica rapa subsp. oleifera |
| 214000 | - |
gel filtration | Brassica rapa subsp. oleifera |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica rapa subsp. oleifera | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Brassica rapa subsp. oleifera |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + pyruvate = ADP + phosphoenolpyruvate | compulsory-ordered tri-bi mechanism | Brassica rapa subsp. oleifera |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Brassica rapa subsp. oleifera | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate | poor substrates: UDP, GDP | Brassica rapa subsp. oleifera | ATP + pyruvate | - |
? | |
| ADP + phosphoenolpyruvate | best nucleoside diphosphate substrate | Brassica rapa subsp. oleifera | ATP + pyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | x * 55000 + 4-x * 57000, SDS-PAGE | Brassica rapa subsp. oleifera |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Brassica rapa subsp. oleifera |
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