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Literature summary for 2.7.1.40 extracted from

  • De Médicis, E.; Laliberté, J.F.; Vass-Marengo, J.
    Purification and properties of pyruvate kinase from Halobacterium cutirubrum (1982), Biochim. Biophys. Acta, 708, 57-67.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
alanine not Halobacterium salinarum
D-ribose 5-phosphate activation Halobacterium salinarum
Monovalent anions activation, in decreasing order of efficiency: Cl-, Br-, NO3- Halobacterium salinarum

General Stability

General Stability Organism
highly unstable at less than 3 M sodium or potassium chloride, cannot be renatured Halobacterium salinarum
optimal stability at pH 6.5 and room temperature Halobacterium salinarum

Inhibitors

Inhibitors Comment Organism Structure
6-phosphogluconate
-
Halobacterium salinarum
fructose 1,6-diphosphate
-
Halobacterium salinarum
L-alanine not Halobacterium salinarum
phosphate
-
Halobacterium salinarum

Metals/Ions

Metals/Ions Comment Organism Structure
K+ requirement Halobacterium salinarum
K+ best activator at optimal conditions, in decreasing order of efficiency: K+, Rb+, Cs+, Na+, NH4+, Li+ Halobacterium salinarum
monovalent cation in decreasing order of efficiency: K+, Rb+, Cs+, Na+, NH4+, Li+ Halobacterium salinarum
additional information no activation by SCN- Halobacterium salinarum
Na+ in decreasing order of efficiency: K+, Rb+, Cs+, Na+, NH4+, Li+ Halobacterium salinarum
NH4+ in decreasing order of efficiency: K+, Rb+, Cs+, Na+, NH4+, Li+ Halobacterium salinarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73000
-
4 * 73000, SDS-PAGE Halobacterium salinarum
300000
-
gel filtration Halobacterium salinarum

Organism

Organism UniProt Comment Textmining
Halobacterium salinarum
-
-
-
Halobacterium salinarum 9
-
-
-

Purification (Commentary)

Purification (Comment) Organism
at room temperature, partial Halobacterium salinarum

Reaction

Reaction Comment Organism Reaction ID
ATP + pyruvate = ADP + phosphoenolpyruvate allosteric enzyme: homotropic Halobacterium salinarum

Renatured (Commentary)

Renatured (Comment) Organism
the enzyme cannot be renatured Halobacterium salinarum

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Halobacterium salinarum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Halobacterium salinarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate positive cooperativity for phosphoenolpyruvate Halobacterium salinarum ATP + pyruvate
-
?
ADP + phosphoenolpyruvate positive cooperativity for phosphoenolpyruvate Halobacterium salinarum 9 ATP + pyruvate
-
?
additional information allosteric enzyme: homotropic Halobacterium salinarum ?
-
?
additional information allosteric enzyme: homotropic Halobacterium salinarum 9 ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 73000, SDS-PAGE Halobacterium salinarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
cold-labile enzyme Halobacterium salinarum
20
-
most stable at Halobacterium salinarum
50
-
45 min, 15% loss of activity Halobacterium salinarum
60
-
t1/2: 10 min Halobacterium salinarum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
stable at Halobacterium salinarum

Cofactor

Cofactor Comment Organism Structure
AMP allosteric effector Halobacterium salinarum