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Literature summary for 2.7.1.40 extracted from

  • Hohn, T.M.; Paznokas, J.L.
    Purification and properties of two isozymes of pyruvate kinase from Mucor racemosus (1987), J. Bacteriol., 169, 3525-3530.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.40

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54400
-
 4 * 54400, isozyme PK1, SDS-PAGE Mucor racemosus
58000
-
 4 * 58000, SDS-PAGE, isozyme PK5 Mucor racemosus
216000
-
 isozyme PK1, PAGE Mucor racemosus
227000
-
 average MW of all 5 isoforms, PAGE Mucor racemosus
239000
-
 isozyme PK5, PAGE Mucor racemosus

Organism

Organism UniProt Comment Textmining
Mucor racemosus
-
 dimorphic phycomycete
-

Purification (Commentary)

Purification (Comment) Organism
two of 5 electrophoretic isoforms: PK1 and PK5 Mucor racemosus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture hyphal and yeast Mucor racemosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Mucor racemosus
1685
-
 isozyme PK5, pH 6.5, 30°C Mucor racemosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
 Mucor racemosus ATP + pyruvate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 54400, isozyme PK1, SDS-PAGE Mucor racemosus
tetramer 4 * 58000, SDS-PAGE, isozyme PK5 Mucor racemosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mucor racemosus