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Literature summary for 2.7.1.40 extracted from

  • Srivastava, L.K.; Baquer, N.Z.
    Purification and properties of rat brain pyruvate kinase (1985), Arch. Biochem. Biophys., 236, 703-713.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 1,6-diphosphate not Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
AMP not Rattus norvegicus
Ca2+ strong at saturating phosphoenolpyruvate concentrations Rattus norvegicus
L-phenylalanine strong Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
phosphoenolpyruvate pH 7.4, 37°C Rattus norvegicus
0.4 0.6 ADP pH 7.4, 37°C Rattus norvegicus
0.4 0.57 phosphoenolpyruvate pH 7.4, 37°C Rattus norvegicus
0.83
-
ADP in the presence of ATP, pH 7.4, 37°C Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
x * 57000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
female Wistar
-
Rattus norvegicus
-
adult albino
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
593
-
pH 7.4, 37°C Rattus norvegicus

Storage Stability

Storage Stability Organism
-15°C, in 20 mM Tris-HCl, pH 6.8, 1 mM MgCl2, 0.1 mM dithiothreitol, 2 months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
Rattus norvegicus ATP + pyruvate
-
?

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
at 0.125 mM phosphoenolpyruvate Rattus norvegicus
7
-
at saturating phosphoenolpyruvate concentration: 1.25 mM Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5.7 8.5 1.25 mM phosphoenolpyruvate, about half-maximal activity at pH 5.7 and 8.5, about 80% of maximal activity at pH 7.4 Rattus norvegicus
6 7.3 0.125 mM phosphoenolpyruvate, about half-maximal activity at pH 6 and pH 7.3 Rattus norvegicus