Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6-diphosphate | activation | Escherichia coli | |
D-glucose 6-phosphate | isozyme PK II | Escherichia coli | |
D-ribose 5-phosphate | activation | Escherichia coli | |
D-ribose 5-phosphate | isozyme PK II | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
4 * 51000, isozyme PK I, SDS-PAGE | Escherichia coli |
56000 | - |
4 * 56000, isozyme PK II, SDS-PAGE | Escherichia coli |
190000 | - |
isozyme PK II | Escherichia coli |
225000 | 240000 | isozyme PK I | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
and strain B | - |
Purification (Comment) | Organism |
---|---|
2 non-interconvertible forms, isozyme PK I: at room temperature | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Escherichia coli | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
0-4°C, in 10 mM Tris, pH 7.5, 1 mM EDTA, 2 mM 2-mercaptoethanol, 1-10 mg isozyme PK I/ml, up to 9 months, in 10 mM Tris, 1 mM EDTA, 2 mM 2-mercaptoethanol, 0.15 M KCl, pH 8, 0.5-1 mg isozyme PK II/ml, up to 6 months | Escherichia coli |
K+ stabilize during storage, NH4+ or Na+ less efficiently | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | positive cooperativity for phosphoenolpyruvate | Escherichia coli | ATP + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 51000, isozyme PK I, SDS-PAGE | Escherichia coli |
tetramer | 4 * 56000, isozyme PK II, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
in 5 mM phosphate buffer, pH 7.5, isozyme PKI: t1/2: more than 1 h, isozyme PKII: t1/2: 2 min | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | activation | Escherichia coli | |
ATP | isozyme PK II | Escherichia coli |