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Literature summary for 2.7.1.40 extracted from

  • Aust, A.; Yun, S.L.; Suelter, C.H.
    Pyruvate kinase from yeast (Saccharomyces cerevisiae) (1975), Methods Enzymol., 42C, 176-182.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic parameters Saccharomyces cerevisiae
0.049 0.1 IDP cosubstrate phosphoenolpyruvate Saccharomyces cerevisiae
0.099
-
phosphoenolpyruvate pH 6.2, 30°C Saccharomyces cerevisiae
0.16
-
ADP pH 6.2, 30°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
K+ requirement Saccharomyces cerevisiae
K+ Km-value: 50 mM in the presence of fructose diphosphate Saccharomyces cerevisiae
Mg2+ requirement Saccharomyces pastorianus
Mg2+ requirement Saccharomyces cerevisiae
Mg2+ Km-value 2 mM in the presence of fructose diphosphate Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
190000
-
-
Saccharomyces pastorianus
209400
-
sedimentation equilibrium method Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + phosphoenolpyruvate Saccharomyces cerevisiae allosteric enzyme ATP + pyruvate
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
Budweiser baker's yeast
-
Saccharomyces pastorianus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
340
-
pH 6.2, 30°C Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
2-4°C, in 90% saturated ammonium sulfate, at least 1 month Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
Saccharomyces pastorianus ATP + pyruvate
-
?
ADP + phosphoenolpyruvate
-
Saccharomyces cerevisiae ATP + pyruvate
-
?
ADP + phosphoenolpyruvate allosteric enzyme Saccharomyces cerevisiae ATP + pyruvate
-
?
IDP + phosphoenolpyruvate
-
Saccharomyces cerevisiae ITP + pyruvate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 50000-52000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Saccharomyces pastorianus
30
-
assay at Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
cold-labile enzyme Saccharomyces cerevisiae
additional information
-
fructose 1,6-diphosphate enhances rate of inactivation, 25% glycerol and/or KCl and MgCl2 protect Saccharomyces cerevisiae