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Literature summary for 2.7.1.40 extracted from

  • Tuominen, F.W.; Bernlohr, R.W.
    Pyruvate kinase of Bacillus licheniformis (1975), Methods Enzymol., 42C, 157-166.
No PubMed abstract available
Search for more literature for 2.7.1.40

Activating Compound

Activating Compound Comment Organism Structure
additional information the initial rate of catalysis is modulated by substrate activation by phosphoenolpyruvate and ADP, activation by AMP and inhibition by ATP, phosphate and carbamoyl phosphate Bacillus licheniformis

General Stability

General Stability Organism
labile enzyme is stabilized in the active state throughout purification by avoiding extensive dilution to less than 40 mg protein/ml, and by inclusion of a stabilizing ligand mixture of phosphoenolpyruvate, phosphate and Mg2+ in supporting buffers if dilution is unavoidable Bacillus licheniformis
MgATP2-, NAD+, NADH, 50% glycerol or sucrose partially stabilizes Bacillus licheniformis

Inhibitors

Inhibitors Comment Organism Structure
ATP in the presence of Mg2+, not Mn2+, reversible by AMP Bacillus licheniformis
Carbamoyl phosphate
-
 Bacillus licheniformis
phosphate
-
 Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 phosphoenolpyruvate pH 7.1, 30°C, in the presence of Mg2+, 7fold lower in the presence of Mn2+ Bacillus licheniformis
0.7
-
 ADP in the presence of Mg2+, pH 7.1, 30°C Bacillus licheniformis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Bacillus licheniformis
Mn2+ requirement Bacillus licheniformis
additional information K-type allosteric properties only in the presence of Mg2+, not Mn2+ Bacillus licheniformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + phosphoenolpyruvate Bacillus licheniformis allosteric enzyme ATP + pyruvate
-
 ?
ADP + phosphoenolpyruvate Bacillus licheniformis A-5 allosteric enzyme ATP + pyruvate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
 A-5
-
Bacillus licheniformis A-5
-
 A-5
-

Purification (Commentary)

Purification (Comment) Organism
partial Bacillus licheniformis

Source Tissue

Source Tissue Comment Organism Textmining
cell culture vegetative Bacillus licheniformis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
210
-
 pH 7.1, 30°C Bacillus licheniformis

Storage Stability

Storage Stability Organism
-20°C, in potassium phosphate buffer, pH 7, MgCl2, phosphoenolpyruvate and glycerol, 5 months Bacillus licheniformis
4°C, ammonium sulfate preparation, at least 5 months Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate positive cooperativity for phosphoenolpyruvate Bacillus licheniformis ATP + pyruvate
-
 ?
ADP + phosphoenolpyruvate allosteric enzyme Bacillus licheniformis ATP + pyruvate
-
 ?
ADP + phosphoenolpyruvate positive cooperativity for phosphoenolpyruvate Bacillus licheniformis A-5 ATP + pyruvate
-
 ?
ADP + phosphoenolpyruvate allosteric enzyme Bacillus licheniformis A-5 ATP + pyruvate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.4
-
 Bacillus licheniformis