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Literature summary for 2.7.1.32 extracted from

  • Sampels, V.; Hartmann, A.; Dietrich, I.; Coppens, I.; Sheiner, L.; Striepen, B.; Herrmann, A.; Lucius, R.; Gupta, N.
    Conditional mutagenesis of a novel choline kinase demonstrates plasticity of phosphatidylcholine biogenesis and gene expression in Toxoplasma gondii (2012), J. Biol. Chem., 287, 16289-16299.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.32

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta cells and in Saccharomyces cerevisiae KS106 cells Toxoplasma gondii

Inhibitors

Inhibitors Comment Organism Structure
dimethylethanolamine
-
 Toxoplasma gondii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.77
-
 choline in 100 mM Tris-HCl (pH 8.5), at 37°C Toxoplasma gondii
0.94
-
 dimethylethanolamine in 100 mM Tris-HCl (pH 8.5), at 37°C Toxoplasma gondii

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Toxoplasma gondii 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 x * 44000, the parasite has two potential 53000 and 44000 Da isoforms, SDS-PAGE Toxoplasma gondii
53000
-
 x * 53000, the parasite has two potential 53000 and 44000 Da isoforms, SDS-PAGE Toxoplasma gondii
70000
-
 x * 70000, full-length enzyme, SDS-PAGE Toxoplasma gondii

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Toxoplasma gondii

Source Tissue

Source Tissue Comment Organism Textmining
tachyzoite
-
 Toxoplasma gondii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + choline
-
 Toxoplasma gondii ADP + phosphocholine
-
 ?
ATP + dimethylethanolamine
-
 Toxoplasma gondii ADP + phosphodimethylethanolamine
-
 ?
ATP + ethanolamine
-
 Toxoplasma gondii ADP + phosphoethanolamine
-
 ?

Subunits

Subunits Comment Organism
? x * 44000, the parasite has two potential 53000 and 44000 Da isoforms, SDS-PAGE Toxoplasma gondii
? x * 53000, the parasite has two potential 53000 and 44000 Da isoforms, SDS-PAGE Toxoplasma gondii
? x * 70000, full-length enzyme, SDS-PAGE Toxoplasma gondii

General Information

General Information Comment Organism
malfunction the parasite compensates for the loss of full-length enzyme by two potential 53- and 44 kDa isoforms Toxoplasma gondii