Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | ChoKalpha mRNA levels are increased in tumour cell lines in terms of their corresponding normal human primary BEC. ChoKalpha is overexpressed with a consequent increase in phosphorylcholine production in lung-cancer cells. | Homo sapiens |
Application | Comment | Organism |
---|---|---|
diagnostics | choline kinase alpha expression is a new prognostic factor that could be used to help identify patients with earlystage non-small-cell lung cancer who might be at high risk of recurrence, and to identify patients with favourable prognosis who could receive less aggressive treatment options or avoid adjuvant systemic treatment. | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MN58b | specific for choline kinase | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + ATP | Homo sapiens | - |
phosphocholine + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
association between gene expression and survival in patients with non-small-cell lung cancer | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
BEC cell | IBEC (CC-2541), primary bronchial epithelial cell | Homo sapiens | - |
HMEC-1 cell | CC-2551, primary mammary epithelial cell | Homo sapiens | - |
lung | cancer tissue, frozen tissue samples are homogenised and lysed in buffer that contained 1.5 mmol/l magnesium chloride, 0.2 mmol/l EDTA, 0.3 M sodium chloride, 25 mmol/l HEPES at pH 7.5, 20 mmol/l beta-glycerophosphate, and 0.1% Triton X-100. Cells are lysed in ice-cold lysis buffer. Nuclei and detergent-insoluble material are removed by centrifugation. | Homo sapiens | - |
NCI-H1299 cell | non-small cell lung cancer epithelial cell line | Homo sapiens | - |
NCI-H460 cell | non-small cell lung cancer epithelial cell line | Homo sapiens | - |
NCI-H510 cell | small cell lung cancer cell line | Homo sapiens | - |
NCI-H82 cell | small cell lung cancer cell line | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
increase in mRNA levels results in an increase in protein expression and ChoK enzymatic activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + ATP | - |
Homo sapiens | phosphocholine + ADP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ChoKalpha | - |
Homo sapiens |
choline kinase | - |
Homo sapiens |
choline kinase alpha | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0001 | - |
H510 cell, 144 h | Homo sapiens | MN58b | |
0.00027 | - |
H82 cell, 144 h | Homo sapiens | MN58b | |
0.0004 | - |
H510 cell, 72 h | Homo sapiens | MN58b | |
0.0008 | - |
H82 cell, 72 h | Homo sapiens | MN58b | |
0.0009 | - |
H1299 cell, 144 h | Homo sapiens | MN58b | |
0.0011 | - |
H460 cell, 144 h | Homo sapiens | MN58b | |
0.0011 | - |
H510 cell, 48 h | Homo sapiens | MN58b | |
0.0019 | - |
H82 cell, 48 h | Homo sapiens | MN58b | |
0.0026 | - |
H460 cell, 72 h | Homo sapiens | MN58b | |
0.0027 | - |
H1299 cell, 72 h | Homo sapiens | MN58b | |
0.0034 | - |
primary HMEC cell, 144 h | Homo sapiens | MN58b | |
0.0042 | - |
primary BEC cell, 144 h | Homo sapiens | MN58b | |
0.00703 | - |
H460 cell, 48 h | Homo sapiens | MN58b | |
0.0103 | - |
H1299 cell, 48 h | Homo sapiens | MN58b | |
0.0183 | - |
primary BEC cell, 72 h | Homo sapiens | MN58b | |
0.0209 | - |
primary HMEC cell, 72 h | Homo sapiens | MN58b | |
0.0405 | - |
primary BEC cell, 48 h | Homo sapiens | MN58b | |
0.0447 | - |
primary HMEC cell, 48 h | Homo sapiens | MN58b |