Application | Comment | Organism |
---|---|---|
drug development | choline kinase is gaining importance as a potential target for anticancer therapy | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
The genomic sequence of the putative human chk-alpha promoter is obtained by performing a BLAST search in National Center for Biotechnology Information using the known promoter sequence of the rat chk-a gene as query sequence. A 2.3-kb region at the 5'-end of the human chk-a gene on chromosome 11, immediately upstream of the translation start site, is found to contain several putative HRE sites with the core sequence RCGTG. Due to its high GC content, this 2.3-kb region is cloned as four smaller overlapping fragments, and formamide (5%) is added in all PCRs. The four overlapping sequences are PCR amplified from human genomic DNA. The PCR products of paired sense and antisense primer sequences used for the cloning of these fragments (Chk) are subcloned into the pCR 2.1 TA vector. The full 2.3-kb promoter region is obtained by appropriate endonuclease digestions and ligation of these four overlapping fragments. Each of the four fragments alone, as well as several subligations of two or three consecutive fragments, and the full-length 2.3-kb chk-a promoter are cloned into the pGL4-basic Luc reporter vector. | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
commentary | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
PC-3 cell | prostate cancer cell | Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
CHK | - |
Homo sapiens |
chk-alpha | - |
Homo sapiens |
choline kinase | - |
Homo sapiens |