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Literature summary for 2.7.1.32 extracted from

  • Malito, E.; Sekulic, N.; Too, W.C.; Konrad, M.; Lavie, A.
    Elucidation of human choline kinase crystal structures in complex with the products ADP or phosphocholine (2006), J. Mol. Biol., 364, 136-151.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.32

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion using 25 mM Tris/HCl (pH 7.5), 25 mM KCl, 0.5 mM dithiothreitol, 0.0005 ml of 0.1 M MgCl2 in 12-20% PEG 3350 with 0.2 M NaF Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
hemicholinium
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 choline in 100 mM Tris-HCl buffer (pH 7.5) containing 100 mM KCl, 10 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.25 mM NADH, 4 units pyruvate kinase, and 5 units lactate dehydrogenase Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 estimated from amino acid sequence Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P35790
-
-

Purification (Commentary)

Purification (Comment) Organism
gluthatione-Sepharose column chromatography, Resource Q column chromatography and Superdex-200 gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + choline
-
 Homo sapiens ADP + O-phosphocholine
-
 ?

Subunits

Subunits Comment Organism
dimer x-ray crystallography Homo sapiens

Synonyms

Synonyms Comment Organism
choline kinase isoform alpha2
-
 Homo sapiens
CKalpha2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
71.5
-
 choline in 100 mM Tris-HCl buffer (pH 7.5) containing 100 mM KCl, 10 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.25 mM NADH, 4 units pyruvate kinase, and 5 units lactate dehydrogenase Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens