Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Plasmodium falciparum |
The RT-PCR-amplified PfCK gene is cloned in pRSET-C, an Escherichia coli expression vector. The host strain is optimized | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H-89 | no activity on choline kinase at 5 mM ATP, decreasing the ATP concentration to 100 microM has no effect on choline kinase | Plasmodium falciparum | |
HC-3 | poor inhibition of 16% and 18% is observed at 50 microM and 150 microM, respectively, at both 100 and 250 microM choline | Plasmodium falciparum | |
hemicholinium-3 | poor inhibition of 16% and 18% at 0.050 mM and 0.15 mM, respectively, not significantly inhibited by hemicholinium-3 up to 0.025 mM | Plasmodium falciparum | |
hexadecyltrimethylammonium bromide | 60% inhibition at 0.1 mM; HDTAB, structural resemblance to hexadecylphosphocholine, exhibits an antimalarial effect and inhibits choline kinase in a dose-dependent manner. For purified protein: 60% inhibition at 100 microM (in presence of 250 microM choline), 60% inhibition at 50 microM (in presence of 100 microM choline). HDTAB may compete with choline for the choline binding site of choline kinase and may offer competitive inhibition with respect to choline. The concentration of HDTAB required to inhibit Plasmodium falciparum growth by 62% was 10times lower than the concentration required to inhibit purified choline kinase by 60% in vitro | Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MgCl2 | assay at | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + ATP | Plasmodium falciparum | choline kinase may have a regulatory role in phosphatidylcholine biosynthesis | phosphocholine + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Plasmodium falciparum | - |
clone NF-54 and wild type | - |
Purification (Comment) | Organism |
---|---|
Ni-nitrilotriacetic acid agarose affinity and gel filtration | Plasmodium falciparum |
overexpressed PfCK protein is purified to homogeneity using Ni-nitrilotriacetic acid agarose affinity and gel filtration chromatography | Plasmodium falciparum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | clone NF-54 | Plasmodium falciparum | - |
additional information | parasites isolated from infected human erythrocytes | Plasmodium falciparum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + choline | - |
Plasmodium falciparum | ADP + phosphocholine | - |
? | |
choline + ATP | choline kinase may have a regulatory role in phosphatidylcholine biosynthesis | Plasmodium falciparum | phosphocholine + ADP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP:choline phosphotransferase | - |
Plasmodium falciparum |
choline kinase | - |
Plasmodium falciparum |
CK | - |
Plasmodium falciparum |
PfCK | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
assay at | Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Plasmodium falciparum |