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Literature summary for 2.7.1.32 extracted from

  • Choubey, V.; Maity, P.; Guha, M.; Kumar, S.; Srivastava, K.; Puri, S.K.; Bandyopadhyay, U.
    Inhibition of Plasmodium falciparum choline kinase by hexadecyltrimethylammonium bromide: a possible antimalarial mechanism (2007), Antimicrob. Agents Chemother., 51, 696-706.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Plasmodium falciparum
The RT-PCR-amplified PfCK gene is cloned in pRSET-C, an Escherichia coli expression vector. The host strain is optimized Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
H-89 no activity on choline kinase at 5 mM ATP, decreasing the ATP concentration to 100 microM has no effect on choline kinase Plasmodium falciparum
HC-3 poor inhibition of 16% and 18% is observed at 50 microM and 150 microM, respectively, at both 100 and 250 microM choline Plasmodium falciparum
hemicholinium-3 poor inhibition of 16% and 18% at 0.050 mM and 0.15 mM, respectively, not significantly inhibited by hemicholinium-3 up to 0.025 mM Plasmodium falciparum
hexadecyltrimethylammonium bromide 60% inhibition at 0.1 mM; HDTAB, structural resemblance to hexadecylphosphocholine, exhibits an antimalarial effect and inhibits choline kinase in a dose-dependent manner. For purified protein: 60% inhibition at 100 microM (in presence of 250 microM choline), 60% inhibition at 50 microM (in presence of 100 microM choline). HDTAB may compete with choline for the choline binding site of choline kinase and may offer competitive inhibition with respect to choline. The concentration of HDTAB required to inhibit Plasmodium falciparum growth by 62% was 10times lower than the concentration required to inhibit purified choline kinase by 60% in vitro Plasmodium falciparum

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2 assay at Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
choline + ATP Plasmodium falciparum choline kinase may have a regulatory role in phosphatidylcholine biosynthesis phosphocholine + ADP
-
?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-
Plasmodium falciparum
-
clone NF-54 and wild type
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Purification (Commentary)

Purification (Comment) Organism
Ni-nitrilotriacetic acid agarose affinity and gel filtration Plasmodium falciparum
overexpressed PfCK protein is purified to homogeneity using Ni-nitrilotriacetic acid agarose affinity and gel filtration chromatography Plasmodium falciparum

Source Tissue

Source Tissue Comment Organism Textmining
cell culture clone NF-54 Plasmodium falciparum
-
additional information parasites isolated from infected human erythrocytes Plasmodium falciparum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + choline
-
Plasmodium falciparum ADP + phosphocholine
-
?
choline + ATP choline kinase may have a regulatory role in phosphatidylcholine biosynthesis Plasmodium falciparum phosphocholine + ADP
-
?

Synonyms

Synonyms Comment Organism
ATP:choline phosphotransferase
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Plasmodium falciparum
choline kinase
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Plasmodium falciparum
CK
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Plasmodium falciparum
PfCK
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Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
assay at Plasmodium falciparum

Cofactor

Cofactor Comment Organism Structure
ATP
-
Plasmodium falciparum