Any feedback?
Literature summary for 2.7.1.32 extracted from
- Choline kinase in Cuscuta reflexa (1972), Biochem. J., 126, 313-324.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Cuscuta reflexa |  |
| AMP | - |
Cuscuta reflexa | |
| ATP | at 4.9 mM and 8.0 mM 30% inhibition; optimum concentration: 3.2-4.0 mM | Cuscuta reflexa | |
| methionine | - |
Cuscuta reflexa | |
| NaCl | 8.7-35 mM: slight activation, 70 mM: inactivation | Cuscuta reflexa | |
| phosphorylcholine | - |
Cuscuta reflexa | |
| serine | - |
Cuscuta reflexa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Cuscuta reflexa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Cuscuta reflexa | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Na+ | 8.7-35 mM: slight activation, 70 mM: inactivation | Cuscuta reflexa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cuscuta reflexa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cuscuta reflexa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 17.9 | - |
- |
Cuscuta reflexa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, enzyme kept frozen for 3 h and thawed, 15 min in contact with ice, complete inactivation, cannot be reactivated | Cuscuta reflexa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + choline | - |
Cuscuta reflexa | ADP + O-phosphocholine | - |
? | |
| GTP + choline | at 50% of the activity with ATP | Cuscuta reflexa | GDP + choline phosphate | - |
? | |
| UTP + choline | at 20% of the activity with ATP | Cuscuta reflexa | UDP + choline phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Cuscuta reflexa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Cuscuta reflexa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | pH 7.5: about 30% of maximum activity, pH 9.0: about 45% of maximum activity | Cuscuta reflexa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
