Literature summary for 2.7.1.30 extracted from

Balogun, E.O.; Inaoka, D.K.; Shiba, T.; Kido, Y.; Tsuge, C.; Nara, T.; Aoki, T.; Honma, T.; Tanaka, A.; Inoue, M.; Matsuoka, S.; Michels, P.A.; Kita, K.; Harada, S.
Molecular basis for the reverse reaction of African human trypanosomes glycerol kinase (2014), Mol. Microbiol., 94, 1315-1329.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with substrates, sitting drop vapor diffusion method, using M HEPES pH 7.5, 11% (v/v) hexane-1,6-diol, 25% (w/v) PEG400	Trypanosoma brucei gambiense

Protein Variants

Protein Variants	Comment	Organism
D20A	about 95% activity compared to the wild type enzyme	Trypanosoma brucei gambiense
E478A	about 25% activity compared to the wild type enzyme	Trypanosoma brucei gambiense
M1A	about 90% activity compared to the wild type enzyme	Trypanosoma brucei gambiense
R22A	about 60% activity compared to the wild type enzyme	Trypanosoma brucei gambiense
R24A	about 30% activity compared to the wild type enzyme	Trypanosoma brucei gambiense
R477A	inactive	Trypanosoma brucei gambiense
T11V	inactive	Trypanosoma brucei gambiense
T12V	less than 5% activity compared to the wild type enzyme	Trypanosoma brucei gambiense
T273V	about 80% activity compared to the wild type enzyme	Trypanosoma brucei gambiense
T276V	inactive	Trypanosoma brucei gambiense
Y3F	about 80% activity compared to the wild type enzyme	Trypanosoma brucei gambiense

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ADP + sn-glycerol 3-phosphate	Trypanosoma brucei gambiense	-	ATP + glycerol	-	r
ATP + glycerol	Trypanosoma brucei gambiense	-	ADP + sn-glycerol 3-phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei gambiense	D3KVM3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + sn-glycerol 3-phosphate	-	Trypanosoma brucei gambiense	ATP + glycerol	-	r
ATP + glycerol	-	Trypanosoma brucei gambiense	ADP + sn-glycerol 3-phosphate	-	r

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Release 2023.1 (January 2023)