Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Trypanosoma brucei brucei |
expressed in Escherichia coli | Trypanosoma vivax |
expressed in Escherichia coli | Trypanosoma congolense |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycerol | inhibitor of the glycerol kinase reverse reaction | Trypanosoma brucei brucei | |
glycerol | inhibitor of the glycerol kinase reverse reaction | Trypanosoma congolense | |
glycerol | inhibitor of the glycerol kinase reverse reaction | Trypanosoma vivax |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
ADP | in 0.1 M MOPS buffer (pH 6.8) containing 1 mM EDTA, 5 mM MgSO4, temperature not specified in the publication | Trypanosoma congolense | |
0.37 | - |
ADP | in 0.1 M MOPS buffer (pH 6.8) containing 1 mM EDTA, 5 mM MgSO4, temperature not specified in the publication | Trypanosoma brucei brucei | |
0.69 | - |
ADP | in 0.1 M MOPS buffer (pH 6.8) containing 1 mM EDTA, 5 mM MgSO4, temperature not specified in the publication | Trypanosoma vivax | |
1.09 | - |
sn-glycerol 3-phosphate | in 0.1 M MOPS buffer (pH 6.8) containing 1 mM EDTA, 5 mM MgSO4, temperature not specified in the publication | Trypanosoma congolense | |
1.13 | - |
sn-glycerol 3-phosphate | in 0.1 M MOPS buffer (pH 6.8) containing 1 mM EDTA, 5 mM MgSO4, temperature not specified in the publication | Trypanosoma brucei brucei | |
1.37 | - |
sn-glycerol 3-phosphate | in 0.1 M MOPS buffer (pH 6.8) containing 1 mM EDTA, 5 mM MgSO4, temperature not specified in the publication | Trypanosoma vivax |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glycosome | - |
Trypanosoma brucei brucei | 20015 | - |
glycosome | - |
Trypanosoma vivax | 20015 | - |
glycosome | - |
Trypanosoma congolense | 20015 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Trypanosoma brucei brucei |
50000 | - |
x * 50000, SDS-PAGE | Trypanosoma vivax |
50000 | - |
x * 50000, SDS-PAGE | Trypanosoma congolense |
54600 | - |
x * 54600, calculated from amino acid sequence | Trypanosoma vivax |
56200 | - |
x * 56200, calculated from amino acid sequence | Trypanosoma congolense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei brucei | Q9NJP9 | - |
- |
Trypanosoma brucei brucei ILTat1.4 | Q9NJP9 | - |
- |
Trypanosoma congolense | Q75T26 | - |
- |
Trypanosoma congolense IL1180 | Q75T26 | - |
- |
Trypanosoma vivax | B0I530 | - |
- |
Trypanosoma vivax IL1392 | B0I530 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Trypanosoma brucei brucei |
nickel affinity column chromatography | Trypanosoma vivax |
nickel affinity column chromatography | Trypanosoma congolense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + sn-glycerol 3-phosphate | - |
Trypanosoma brucei brucei | ATP + glycerol | - |
r | |
ADP + sn-glycerol 3-phosphate | - |
Trypanosoma vivax | ATP + glycerol | - |
r | |
ADP + sn-glycerol 3-phosphate | - |
Trypanosoma congolense | ATP + glycerol | - |
r | |
ADP + sn-glycerol 3-phosphate | - |
Trypanosoma congolense IL1180 | ATP + glycerol | - |
r | |
ADP + sn-glycerol 3-phosphate | - |
Trypanosoma brucei brucei ILTat1.4 | ATP + glycerol | - |
r | |
ADP + sn-glycerol 3-phosphate | - |
Trypanosoma vivax IL1392 | ATP + glycerol | - |
r | |
ATP + glycerol | - |
Trypanosoma brucei brucei | ADP + sn-glycerol 3-phosphate | - |
r | |
ATP + glycerol | - |
Trypanosoma vivax | ADP + sn-glycerol 3-phosphate | - |
r | |
ATP + glycerol | - |
Trypanosoma congolense | ADP + sn-glycerol 3-phosphate | - |
r | |
ATP + glycerol | - |
Trypanosoma congolense IL1180 | ADP + sn-glycerol 3-phosphate | - |
r | |
ATP + glycerol | - |
Trypanosoma brucei brucei ILTat1.4 | ADP + sn-glycerol 3-phosphate | - |
r | |
ATP + glycerol | - |
Trypanosoma vivax IL1392 | ADP + sn-glycerol 3-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Trypanosoma brucei brucei |
? | x * 50000, SDS-PAGE | Trypanosoma vivax |
? | x * 50000, SDS-PAGE | Trypanosoma congolense |
? | x * 56200, calculated from amino acid sequence | Trypanosoma congolense |
? | x * 54600, calculated from amino acid sequence | Trypanosoma vivax |