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Literature summary for 2.7.1.30 extracted from

  • Pettigrew, D.W.
    Amino acid substitutions in the sugar kinase/hsp70/actin superfamily conserved ATPase core of E. coli glycerol kinase modulate allosteric ligand affinity but do not alter allosteric coupling (2009), Arch. Biochem. Biophys., 481, 151-156.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.30

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
E478C mutation increases the affinity for glucose-specific phosphocarrier protein of the phosphoenolpyruvate:glucose phosphotransferase system (IIA(Glc)) Escherichia coli
E478C/T428V/R429N T428V and R429N replace two coupling locus amino acids with those from Haemophilus influenzae glycerol kinase Escherichia coli
G427D/T428V/R429N replacement of all three of the coupling locus amino acids with those from Haemophilus influenzae glycerol kinase Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
glucose-specific phosphocarrier protein of the phosphoenolpyruvate:glucose phosphotransferase system (IIA(Glc)) allosteric inhibitor Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 ATP E478C mutant protein, allosteric regulation Escherichia coli
0.009
-
 ATP ATP in form of MgATP2- Escherichia coli
0.01
-
 ATP E478C/T428V/R429N mutant protein, allosteric regulation Escherichia coli
0.01
-
 ATP ATP in form of MgATP2- Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glycerol
-
 Escherichia coli ADP + sn-glycerol 3-phosphate
-
 ?
glycerol + ATP
-
 Escherichia coli sn-glycerol 3-phosphate + ADP
-
 ?

Synonyms

Synonyms Comment Organism
ATP:glycerol 3-phosphotransferase
-
 Escherichia coli
glycerol kinase
-
 Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.001
-
 E478C mutant protein Escherichia coli glucose-specific phosphocarrier protein of the phosphoenolpyruvate:glucose phosphotransferase system (IIA(Glc))
0.001
-
 in the presence of ZnCl2 Escherichia coli glucose-specific phosphocarrier protein of the phosphoenolpyruvate:glucose phosphotransferase system (IIA(Glc))
0.01
-
 wild-type protein Escherichia coli glucose-specific phosphocarrier protein of the phosphoenolpyruvate:glucose phosphotransferase system (IIA(Glc))