Literature summary for 2.7.1.30 extracted from

Matsumura, K.; Chang, B.H.; Fujimiya, M.; Chen, W.; Kulkarni, R.N.; Eguchi, Y.; Kimura, H.; Kojima, H.; Chan, L.
Aquaporin 7 Is a beta-cell protein and regulator of intraislet glycerol content and glycerol kinase activity, beta-cell mass, and insulin production and secretion (2007), Mol. Cell. Biol., 27, 6026-6037.

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Protein Variants

Protein Variants	Comment	Organism
additional information	Aqp7-/- knock out mice. Gene replacement targeting strategy is used to inactivate the Aqp7 gene in R1 mouse ES cells. Targeted ES cell clones are injected into blastocysts of C57BL/6J mice. Glycerol kinase expression (mRNA level) and activity is increased in pancreatic islets of Aqp7-/- mice compared to Aqp+/+ mice.	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	wild type and Aqp7 knock out mice (Aqp7-/-), 14- to 18-week-old animals	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
pancreas	pancreatic islets and pancreas. The glycerol kinase mRNA level in the Aqp7-/- pancreatic islets is 655% that in Aqp7+/+ islets. The glycerol activity is increased in both total pancreases and islets isolated from Aqp7-/- mice compared with those isolated from Aqp+/+ mice (tested with cell homogenates, activity assay: 50 mM Tris-HCl, pH 7.2, 5 mM ATP, 10 mM MgCl2, 100 mM KCl, 2.5 mM dithiothreitol, 4 mM glycerol, 50 microM [14C]glycerol, for 3 h at 37°C). The absence of AQP7 is associated with a moderate increase in the glycerol content of the total pancreas. There is a 2fold increase in the glycerol content of pancreatic islets isolated from Aqp7-/- mice compared with those from Aqp7+/+ mice.	Mus musculus	-

Synonyms

Synonyms	Comment	Organism
GYK	glycerol kinase	Mus musculus

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Release 2023.1 (January 2023)