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Literature summary for 2.7.1.30 extracted from

  • Vahedi-Faridi, A.; Stojanoff, V.; Yeh, J.I.
    The effects of flash-annealing on glycerol kinase crystals (2005), Acta Crystallogr. Sect. D, 61, 982-989.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals are obtained by the hanging-drop technique from a solution containing 29% polyethylene glycol 400, 0.1 M sodium acetate pH 4.5, 0.1 M calcium acetate and 10% glycerol. The crystals can grow in the presence of 33% PEG 400, which allows to mount the crystals and directyl flash-cool them. Repeated flash-annealing causes a significant decrease in the averaged mosaicity along with an increase in the overall peak counts of reflections and an enhanced signal-to-noise ratio. Individual reflection-profile analysis reveales a mostly dual domain structure, showing the minimization of one domain as a result of flash-annealing. Enterococcus casseliflavus

Organism

Organism UniProt Comment Textmining
Enterococcus casseliflavus
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