Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
General Stability | Organism |
---|---|
glycerol, 0.01 M, EDTA, 0.001 M and 0.001 M 2-mercaptoethanol prevent inactivation during purification | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fructose 1,6-diphosphate | - |
Escherichia coli | |
L-alpha-glycerophosphate | no inhibition up to 3 mM in presence of 0.1 M glycerol | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli | |
0.5 | - |
dihydroxyacetone | 25°C, plus D-glyceraldehyde | Escherichia coli | |
3 | - |
L-glyceraldehyde | 25°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli | |
Mn2+ | can substitute for Mg2+ | Escherichia coli | |
Mn2+ | less active at equal molar concentration than Mg2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
280000 | 300000 | sedimentation equilibrium light scattering method | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
100 | - |
25°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glyceraldehyde | - |
Escherichia coli | ADP + D-glyceraldehyde 3-phosphate | - |
? | |
ATP + dihydroxyacetone | - |
Escherichia coli | ADP + dihydroxyacetone phosphate | - |
? | |
ATP + glycerol | high specificity for ATP, no other ribonucleoside triphosphate utilized | Escherichia coli | ADP + sn-glycerol 3-phosphate | i.e. L-alpha-glycerophosphate | ? | |
ATP + L-glyceraldehyde | - |
Escherichia coli | ADP + L-glyceraldehyde 3-phosphate | - |
? | |
additional information | D-glyceraldehyde promotes conversion of ATP to ADP + phosphate | Escherichia coli | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.8 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 10.5 | pH 6.8: about 30% of maximum activity, pH 10.5: about 80% of maximum activity | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | 0°C, half-life: 24 h | Escherichia coli |