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Literature summary for 2.7.1.30 extracted from

  • Thorner, J.W.
    Glycerol kinase (1975), Methods Enzymol., 42C, 148-156.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
Escherichia coli
fructose 1,6-diphosphate
-
Escherichia coli
p-Hydroxymercuriphenylsulfonate
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information assay at 25°C, increasing pH or decreasing temperature rises Km for glycerol Escherichia coli
0.5
-
dihydroxyacetone pH 7.0, 25°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
KCl 0.04 M, stimulates 30% at neutral pH Escherichia coli
Mg2+ true substrate: MgATP-complex Escherichia coli
Mn2+ can substitute for Mg2+ Escherichia coli
Mn2+ rate is 3-fold lower than with Mg2+ Escherichia coli
additional information no effect: Ca2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
4 * 55000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
41.2
-
pH 7.0, 25°C Escherichia coli

Storage Stability

Storage Stability Organism
0°C, suspension of crystals, 10 mM glycerol, 1 mM EDTA, 1 mM 2-mercaptoethanol, 0.1 M potassium phosphate, pH 7.0, saturated with ammonium sulfate, stable for several years Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glyceraldehyde
-
Escherichia coli ADP + D-glyceraldehyde 3-phosphate
-
?
ATP + dihydroxyacetone
-
Escherichia coli ADP + dihydroxyacetone phosphate
-
?
ATP + glycerol high specificity for ATP, no other ribonucleoside triphosphate utilized Escherichia coli ADP + sn-glycerol 3-phosphate
-
ir
ATP + L-glyceraldehyde
-
Escherichia coli ADP + L-glyceraldehyde 3-phosphate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 55000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 pH 7.0: about 50% of maximum activity, pH 9.5: maximum activity Escherichia coli