Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Escherichia coli | |
fructose 1,6-diphosphate | - |
Escherichia coli | |
p-Hydroxymercuriphenylsulfonate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | assay at 25°C, increasing pH or decreasing temperature rises Km for glycerol | Escherichia coli | |
0.5 | - |
dihydroxyacetone | pH 7.0, 25°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | 0.04 M, stimulates 30% at neutral pH | Escherichia coli | |
Mg2+ | true substrate: MgATP-complex | Escherichia coli | |
Mn2+ | can substitute for Mg2+ | Escherichia coli | |
Mn2+ | rate is 3-fold lower than with Mg2+ | Escherichia coli | |
additional information | no effect: Ca2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
4 * 55000, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
41.2 | - |
pH 7.0, 25°C | Escherichia coli |
Storage Stability | Organism |
---|---|
0°C, suspension of crystals, 10 mM glycerol, 1 mM EDTA, 1 mM 2-mercaptoethanol, 0.1 M potassium phosphate, pH 7.0, saturated with ammonium sulfate, stable for several years | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glyceraldehyde | - |
Escherichia coli | ADP + D-glyceraldehyde 3-phosphate | - |
? | |
ATP + dihydroxyacetone | - |
Escherichia coli | ADP + dihydroxyacetone phosphate | - |
? | |
ATP + glycerol | high specificity for ATP, no other ribonucleoside triphosphate utilized | Escherichia coli | ADP + sn-glycerol 3-phosphate | - |
ir | |
ATP + L-glyceraldehyde | - |
Escherichia coli | ADP + L-glyceraldehyde 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 55000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | pH 7.0: about 50% of maximum activity, pH 9.5: maximum activity | Escherichia coli |