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Literature summary for 2.7.1.21 extracted from

  • Mutahir, Z.; Larsen, N.B.; Christiansen, L.S.; Andersson, K.M.; Rico, R.; Wisen, S.M.; Clausen, A.R.; Munch-Petersen, B.; Piskur, J.
    Characterization of oligomeric and kinetic properties of tomato thymidine kinase 1 (2011), Nucleosides Nucleotides Nucleic Acids, 30, 1223-1226.
    View publication on PubMed

Application

Application Comment Organism
medicine the thymidine kinase 1 gene from tomato in combination with azidothymidine is a powerful suicide gene for anticancer gene therapy Solanum lycopersicum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli KY895 cells Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25600
-
2 * 25600, calculated from amino acid sequence Solanum lycopersicum
38000
-
thymidine kinase 1 without ATP, gel filtration Solanum lycopersicum
59000
-
thymidine kinase 1 with ATP, gel filtration Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum Q71F77
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + azidothymidine
-
Solanum lycopersicum ?
-
?
ATP + azidothymidine monophosphate
-
Solanum lycopersicum ?
-
?
ATP + thymidine
-
Solanum lycopersicum ADP + dTMP
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 25600, calculated from amino acid sequence Solanum lycopersicum

Synonyms

Synonyms Comment Organism
thymidine kinase 1
-
Solanum lycopersicum
TK1
-
Solanum lycopersicum