Literature summary for 2.7.1.21 extracted from

Welin, M.; Kosinska, U.; Mikkelsen, N.E.; Carnrot, C.; Zhu, C.; Wang, L.; Eriksson, S.; Munch-Petersen, B.; Eklund, H.
Structures of thymidine kinase 1 of human and mycoplasmic origin (2004), Proc. Natl. Acad. Sci. USA, 101, 17970-17975.

Application

Application	Comment	Organism
medicine	enzyme can be useful in gene therapy	Ureaplasma urealyticum
medicine	enzyme can be useful in gene therapy	Homo sapiens
synthesis	enzyme can be useful in drug design	Ureaplasma urealyticum
synthesis	enzyme can be useful in drug design	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression of C-terminally truncated enzyme lacking 41 amino acid residues	Homo sapiens
gene tdk, expression in Escherichia coli as wild-type and selenomethionine-labeled enzymes	Ureaplasma urealyticum

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant C-terminally truncated enzyme in complex with inhibitor dTTP, hanging drop vapour diffusion method, 7-13 mg/ml protein in solution with 5 mM dTTP mixed with an equal volume of crystallization solution containing 0.1 M Na-citrate, pH 5.6, 20% 2-propanol, and 14-16% PEG 4000, 15°C, X-ray diffraction structure determination and analysis at 2.4-3.5 A resolution	Homo sapiens
purified recombinant wild-type and selenomethionine-labeled enzyme in complex with inhibitor dTTP, hanging drop vapour diffusion method, 8 mg/ml protein in solution with 5 mM dTTp mixed with an equal volume of reservoir solution containing 19% PEG 2000, 0.2 M LiCl, 5 mM DTT, and 0.1 M MES, pH 6.0, 15°C, X-ray diffraction structure determination and analysis at 2.5 or 3.0 A resolution, respectively	Ureaplasma urealyticum

Crystallization (Comment)

Organism

purified recombinant C-terminally truncated enzyme in complex with inhibitor dTTP, hanging drop vapour diffusion method, 7-13 mg/ml protein in solution with 5 mM dTTP mixed with an equal volume of crystallization solution containing 0.1 M Na-citrate, pH 5.6, 20% 2-propanol, and 14-16% PEG 4000, 15°C, X-ray diffraction structure determination and analysis at 2.4-3.5 A resolution

Homo sapiens

purified recombinant wild-type and selenomethionine-labeled enzyme in complex with inhibitor dTTP, hanging drop vapour diffusion method, 8 mg/ml protein in solution with 5 mM dTTp mixed with an equal volume of reservoir solution containing 19% PEG 2000, 0.2 M LiCl, 5 mM DTT, and 0.1 M MES, pH 6.0, 15°C, X-ray diffraction structure determination and analysis at 2.5 or 3.0 A resolution, respectively

Ureaplasma urealyticum

Inhibitors

Inhibitors	Comment	Organism	Structure
dTTP	competitive feedback inhibition, binding structure analysis	Homo sapiens
dTTP	competitive feedback inhibition, binding structure analysis	Ureaplasma urealyticum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Ureaplasma urealyticum	5829	-
cytosol	-	Homo sapiens	5829	-

Metals/Ions

Metals/Ions	Comment	Organism
Mg2+	-	Ureaplasma urealyticum
Mg2+	-	Homo sapiens
Zn2+	at a molar ratio of 0.51, connects beta-structures at the root of a ribbon that forms a stem which widens to a lasso-loop	Ureaplasma urealyticum
Zn2+	at a molar ratio of 0.56, connects beta-structures at the root of a ribbon that forms a stem which widens to a lasso-loop	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
ATP + 2'-deoxythymidine	Ureaplasma urealyticum	-	ADP + 2'-deoxythymidine 5'-phosphate	-	ir
ATP + 2'-deoxythymidine	Homo sapiens	-	ADP + 2'-deoxythymidine 5'-phosphate	-	ir
ATP + 2'-deoxyuridine	Ureaplasma urealyticum	-	ADP + 2'-deoxyuridine 5'-phosphate	-	?
ATP + 2'-deoxyuridine	Homo sapiens	-	ADP + 2'-deoxyuridine 5'-phosphate	-	?
additional information	Homo sapiens	the cell-cycle-regulated enzyme is important in nucleotide metabolism and an activator of antiviral and anticancer drugs	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P04183	-	-
Ureaplasma urealyticum	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + thymidine = ADP + dTMP	substrate binding and active site structure	Ureaplasma urealyticum	a
ATP + thymidine = ADP + dTMP	substrate binding and active site structure	Homo sapiens	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + 2'-deoxythymidine	-	Ureaplasma urealyticum	ADP + 2'-deoxythymidine 5'-phosphate	-	ir
ATP + 2'-deoxythymidine	-	Homo sapiens	ADP + 2'-deoxythymidine 5'-phosphate	-	ir
ATP + 2'-deoxyuridine	-	Ureaplasma urealyticum	ADP + 2'-deoxyuridine 5'-phosphate	-	?
ATP + 2'-deoxyuridine	-	Homo sapiens	ADP + 2'-deoxyuridine 5'-phosphate	-	?
additional information	the cell-cycle-regulated enzyme is important in nucleotide metabolism and an activator of antiviral and anticancer drugs	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
tetramer	each subunit contains an alpha/beta-domain, and a domain with a structural zinc ion	Ureaplasma urealyticum
tetramer	each subunit contains an alpha/beta-domain, and a domain with a structural zinc ion	Homo sapiens

Synonyms

Synonyms	Comment	Organism
More	the enzyme belongs to the thymidine kinase 1, i.e. TK1, family of enzymes	Ureaplasma urealyticum
More	the enzyme belongs to the thymidine kinase 1, i.e. TK1, family of enzymes	Homo sapiens
thymidine kinase 1	-	Ureaplasma urealyticum
thymidine kinase 1	-	Homo sapiens
TK1	-	Ureaplasma urealyticum
TK1	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Ureaplasma urealyticum
ATP	-	Homo sapiens