Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.21 extracted from

  • Birringer, M.S.; Claus, M.T.; Folkers, G.; Kloer, D.P.; Schulz, G.E.; Scapozza, L.
    Structure of a type II thymidine kinase with bound dTTP (2005), FEBS Lett., 579, 1376-1382.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
N-terminal thrombin-cleavable His6-tagged enzyme, missing 14 amino acids of the N-terminus and 40 amino acids of the C-terminus, in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant truncated enzyme comprising residues 15-194 of the wild-type enzyme without His-tag, hanging drop vapour diffusion method, 7 mg/ml protein in 5 mM Tris-HCl, pH 7.2, 10 mM NaCl, and 10 mM DTT, mixed at equal volumes with crystallization buffer containing 0.095 mM tri-sodium citrate, pH 5.5, 12% PEG 4000, 10% isopropanol, 23°C, equilibration against a reservoir of 0.5 ml crystallization buffer, 3 days, X-ray diffraction structure determination and analysis at 1.7 A resolution Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
dTTP feedback inhibitor, binds tightly to the enzyme at the active site Homo sapiens
dTTP binds to the active site Leishmania major
dTTP binds to the active site Mycoplasma pneumoniae
dTTP binds to the active site Vaccinia virus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Leishmania major 5829
-
cytosol
-
Homo sapiens 5829
-
cytosol
-
Mycoplasma pneumoniae 5829
-
cytosol
-
Vaccinia virus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Leishmania major
Mg2+
-
Homo sapiens
Mg2+
-
Mycoplasma pneumoniae
Mg2+
-
Vaccinia virus
Zn2+ Cys residues C153, C156, C185, and C188 are involved in binding Homo sapiens
Zn2+ enzyme contains a zinc finger binding motif Leishmania major
Zn2+ enzyme contains a zinc finger binding motif Mycoplasma pneumoniae
Zn2+ enzyme contains a zinc finger binding motif Vaccinia virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2'-deoxythymidine Leishmania major
-
ADP + 2'-deoxythymidine 5'-phosphate
-
?
ATP + 2'-deoxythymidine Homo sapiens
-
ADP + 2'-deoxythymidine 5'-phosphate
-
?
ATP + 2'-deoxythymidine Mycoplasma pneumoniae
-
ADP + 2'-deoxythymidine 5'-phosphate
-
?
ATP + 2'-deoxythymidine Vaccinia virus
-
ADP + 2'-deoxythymidine 5'-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P04183
-
-
Leishmania major
-
-
-
Mycoplasma pneumoniae P75070
-
-
Vaccinia virus O57203
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + thymidine = ADP + dTMP active site structure Leishmania major
ATP + thymidine = ADP + dTMP active site structure Mycoplasma pneumoniae
ATP + thymidine = ADP + dTMP active site structure Vaccinia virus
ATP + thymidine = ADP + dTMP active site structure, E98 is involved in catalysis Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxythymidine
-
Leishmania major ADP + 2'-deoxythymidine 5'-phosphate
-
?
ATP + 2'-deoxythymidine
-
Homo sapiens ADP + 2'-deoxythymidine 5'-phosphate
-
?
ATP + 2'-deoxythymidine
-
Mycoplasma pneumoniae ADP + 2'-deoxythymidine 5'-phosphate
-
?
ATP + 2'-deoxythymidine
-
Vaccinia virus ADP + 2'-deoxythymidine 5'-phosphate
-
?
additional information narrow substrate specificity Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
dimer the truncated, dimeric enzyme form is fully active Homo sapiens
tetramer subunit arrangement of the tetrameric enzyme, enzyme contains a zinc finger domain at residues 150-191 Homo sapiens

Synonyms

Synonyms Comment Organism
TK1
-
Leishmania major
TK1
-
Homo sapiens
TK1
-
Mycoplasma pneumoniae
TK1
-
Vaccinia virus
type II thymidine kinase 1
-
Leishmania major
type II thymidine kinase 1
-
Homo sapiens
type II thymidine kinase 1
-
Mycoplasma pneumoniae
type II thymidine kinase 1
-
Vaccinia virus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Leishmania major
ATP
-
Homo sapiens
ATP
-
Mycoplasma pneumoniae
ATP
-
Vaccinia virus