Cloned (Comment) | Organism |
---|---|
N-terminal thrombin-cleavable His6-tagged enzyme, missing 14 amino acids of the N-terminus and 40 amino acids of the C-terminus, in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant truncated enzyme comprising residues 15-194 of the wild-type enzyme without His-tag, hanging drop vapour diffusion method, 7 mg/ml protein in 5 mM Tris-HCl, pH 7.2, 10 mM NaCl, and 10 mM DTT, mixed at equal volumes with crystallization buffer containing 0.095 mM tri-sodium citrate, pH 5.5, 12% PEG 4000, 10% isopropanol, 23°C, equilibration against a reservoir of 0.5 ml crystallization buffer, 3 days, X-ray diffraction structure determination and analysis at 1.7 A resolution | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dTTP | feedback inhibitor, binds tightly to the enzyme at the active site | Homo sapiens | |
dTTP | binds to the active site | Leishmania major | |
dTTP | binds to the active site | Mycoplasma pneumoniae | |
dTTP | binds to the active site | Vaccinia virus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Leishmania major | 5829 | - |
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | - |
Mycoplasma pneumoniae | 5829 | - |
cytosol | - |
Vaccinia virus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Leishmania major | |
Mg2+ | - |
Homo sapiens | |
Mg2+ | - |
Mycoplasma pneumoniae | |
Mg2+ | - |
Vaccinia virus | |
Zn2+ | Cys residues C153, C156, C185, and C188 are involved in binding | Homo sapiens | |
Zn2+ | enzyme contains a zinc finger binding motif | Leishmania major | |
Zn2+ | enzyme contains a zinc finger binding motif | Mycoplasma pneumoniae | |
Zn2+ | enzyme contains a zinc finger binding motif | Vaccinia virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxythymidine | Leishmania major | - |
ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 2'-deoxythymidine | Homo sapiens | - |
ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 2'-deoxythymidine | Mycoplasma pneumoniae | - |
ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 2'-deoxythymidine | Vaccinia virus | - |
ADP + 2'-deoxythymidine 5'-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P04183 | - |
- |
Leishmania major | - |
- |
- |
Mycoplasma pneumoniae | P75070 | - |
- |
Vaccinia virus | O57203 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + thymidine = ADP + dTMP | active site structure | Leishmania major | |
ATP + thymidine = ADP + dTMP | active site structure | Mycoplasma pneumoniae | |
ATP + thymidine = ADP + dTMP | active site structure | Vaccinia virus | |
ATP + thymidine = ADP + dTMP | active site structure, E98 is involved in catalysis | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxythymidine | - |
Leishmania major | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 2'-deoxythymidine | - |
Homo sapiens | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 2'-deoxythymidine | - |
Mycoplasma pneumoniae | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 2'-deoxythymidine | - |
Vaccinia virus | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
additional information | narrow substrate specificity | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | the truncated, dimeric enzyme form is fully active | Homo sapiens |
tetramer | subunit arrangement of the tetrameric enzyme, enzyme contains a zinc finger domain at residues 150-191 | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
TK1 | - |
Leishmania major |
TK1 | - |
Homo sapiens |
TK1 | - |
Mycoplasma pneumoniae |
TK1 | - |
Vaccinia virus |
type II thymidine kinase 1 | - |
Leishmania major |
type II thymidine kinase 1 | - |
Homo sapiens |
type II thymidine kinase 1 | - |
Mycoplasma pneumoniae |
type II thymidine kinase 1 | - |
Vaccinia virus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Leishmania major | |
ATP | - |
Homo sapiens | |
ATP | - |
Mycoplasma pneumoniae | |
ATP | - |
Vaccinia virus |