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Literature summary for 2.7.1.21 extracted from
- Kinetic analysis and ligand-induced conformational changes in dimeric and tetrameric forms of human thymidine kinase 2 (2005), Biochemistry, 44, 4886-4896.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination, expression in Escherichia coli as maltose binding fusion protein in inclusion bodies, coexpression of GroEL/ES molecular chaperone system and 3% v/v ethanol supplementation | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| binding of dNTP inhibitors and substrates, i.e. 2'-deoxycytidine, Mg-2'-deoxyCTP, 2'-deoxythymidine, and Mg-2'-deoxyTTP structurally stabilizes the enzyme, ligand-bound enzyme is less susceptible to aggregation, proteolysis by trypsin, and thermal denaturation | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dATP | strong feedback inhibition, tight binding at the active site in stoichiometric amount | Homo sapiens |  |
| dCTP | strong feedback inhibition, tight binding at the active site in stoichiometric amount | Homo sapiens | |
| dTTP | strong feedback inhibition, tight binding at the active site in stoichiometric amount | Homo sapiens | |
| additional information | inhibition potency of dNTPs in descending order: dTTP, dCTP, dATP, inhibitor binding structurally stabilizes the enzyme, induction of conformational changes upon ligand binding, overview | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics, kinetics of conformational changes induced by ligand binding | Homo sapiens | |
| 0.0028 | - |
ATP | dNTP-free recombinant dimeric enzyme, pH 7.5, 25°C | Homo sapiens | |
| 0.0028 | - |
ATP | ATP in form of MgATP2- | Homo sapiens | |
| 0.004 | - |
2'-deoxycytidine | dNTP-free recombinant dimeric enzyme, pH 7.5, 25°C | Homo sapiens | |
| 0.0049 | - |
2'-deoxycytidine | dNTP-free recombinant tetrameric enzyme, pH 7.5, 25°C | Homo sapiens | |
| 0.0058 | - |
2'-deoxythymidine | dNTP-free recombinant dimeric enzyme, pH 7.5, 25°C | Homo sapiens | |
| 0.0059 | - |
ATP | dNTP-free recombinant tetrameric enzyme, pH 7.5, 25°C | Homo sapiens | |
| 0.0059 | - |
ATP | ATP in form of MgATP2- | Homo sapiens | |
| 0.0065 | - |
2'-deoxythymidine | dNTP-free recombinant tetrameric enzyme, pH 7.5, 25°C | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2'-deoxycytidine | Homo sapiens | - |
ADP + 2'-deoxycytidine 5'-phosphate | - |
ir | |
| ATP + 2'-deoxythymidine | Homo sapiens | - |
ADP + 2'-deoxythymidine 5'-phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme as maltose binding fusion protein from Escherichia coli | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2'-deoxycytidine | - |
Homo sapiens | ADP + 2'-deoxycytidine 5'-phosphate | - |
ir | |
| ATP + 2'-deoxycytidine | induction of conformational changes upon ligand binding, overview | Homo sapiens | ADP + 2'-deoxycytidine 5'-phosphate | - |
ir | |
| ATP + 2'-deoxythymidine | - |
Homo sapiens | ADP + 2'-deoxythymidine 5'-phosphate | - |
ir | |
| ATP + 2'-deoxythymidine | induction of conformational changes upon ligand binding, overview | Homo sapiens | ADP + 2'-deoxythymidine 5'-phosphate | - |
ir | |
| ATP + thymidine | - |
Homo sapiens | ADP + dTMP | - |
? | |
| additional information | ligand-induced conformational changes in dimeric and tetrameric forms of the enzyme alter the catalytic activity | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | the most abundant enzyme form, less active than the tetramer | Homo sapiens |
| More | ligand-induced conformational changes in dimeric and tetrameric forms of the enzyme | Homo sapiens |
| tetramer | less abundant form, more active than the dimer | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the thymidine kinase 2, i.e. TK2, family of enzymes | Homo sapiens |
| thymidine kinase 2 | - |
Homo sapiens |
| TK2 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
irreversible thermal denaturation following a two-state irrversible denaturation model, study with ligand-bound and free enzyme, respectively, overview | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| MgATP2- | - |
Homo sapiens | |
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