Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination, expression in Escherichia coli as maltose binding fusion protein in inclusion bodies, coexpression of GroEL/ES molecular chaperone system improves enzyme solubility only marginally, but ethanol supplementation contributes to enzyme recovery due to activation of heat shock response | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Homo sapiens | |
0.0047 | - |
ATP | dimeric enzyme, pH 7.5, 25°C | Homo sapiens | |
0.0109 | - |
ATP | tetrameric enzyme, pH 7.5, 25°C | Homo sapiens | |
0.018 | - |
2'-deoxycytidine | tetrameric enzyme, pH 7.5, 25°C | Homo sapiens | |
0.025 | - |
2'-deoxycytidine | dimeric enzyme, pH 7.5, 25°C | Homo sapiens | |
0.05 | - |
2'-deoxythymidine | tetrameric enzyme, pH 7.5, 25°C | Homo sapiens | |
0.054 | - |
2'-deoxythymidine | dimeric enzyme, pH 7.5, 25°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
oligomeric states have MWs of 152200 and 95900, as determined by gel filtration | Homo sapiens |
48800 | - |
recombinant enzyme, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxycytidine | Homo sapiens | - |
ADP + 2'-deoxycytidine 5'-phosphate | - |
ir | |
ATP + 2'-deoxythymidine | Homo sapiens | - |
ADP + 2'-deoxythymidine 5'-phosphate | - |
ir | |
ATP + 2'-deoxyuridine | Homo sapiens | - |
ADP + 2'-deoxyuridine 5'-phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O00142 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme as maltose binding fusion protein from Escherichia coli | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + thymidine = ADP + dTMP | molecular docking of substrates and inhibitors, active site structure modeling | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
coexpression of GroEL/ES molecular chaperone system improves recombinant enzyme solubility from inclusion bodies only marginally, but ethanol supplementation contributes to enzyme recovery due to activation of heat shock response | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | expression in nonproliferating tissue | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxycytidine | - |
Homo sapiens | ADP + 2'-deoxycytidine 5'-phosphate | - |
ir | |
ATP + 2'-deoxythymidine | - |
Homo sapiens | ADP + 2'-deoxythymidine 5'-phosphate | - |
ir | |
ATP + 2'-deoxyuridine | - |
Homo sapiens | ADP + 2'-deoxyuridine 5'-phosphate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | enzyme appears as dimer and tetramer | Homo sapiens |
More | structure modeling | Homo sapiens |
tetramer | enzyme appears as dimer and tetramer | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
2'-deoxythymidine kinase | - |
Homo sapiens |
More | the enzyme belongs to the thymidine kinase 2, i.e. TK2, family of enzymes | Homo sapiens |
thymidine kinase 2 | - |
Homo sapiens |
TK2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 37 | highest activity within this temperature range at 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |