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Literature summary for 2.7.1.21 extracted from

  • Amrhein, I.; Wurth, C.; Bohner, T.; Hofbauer, R.; Folkers, G.; Scapozza, L.
    Highly purified recombinant varicella Zoster virus thymidine kinase is a homodimer (2000), Protein Expr. Purif., 18, 338-345.
    View publication on PubMed
Search for more literature for 2.7.1.21

Cloned(Commentary)

Cloned (Comment) Organism
PreScission-cleavable glutathione S-transferase fusion protein Human alphaherpesvirus 3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
 thymidine 37°C Human alphaherpesvirus 3
0.01163
-
 ATP 37°C Human alphaherpesvirus 3

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70400 80000 gel filtration Human alphaherpesvirus 3
77100 80700 native PAGE Human alphaherpesvirus 3

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + thymidine Human alphaherpesvirus 3
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Human alphaherpesvirus 3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
expressed in Escherichia coli, homogeneity Human alphaherpesvirus 3

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
655
-
-
 Human alphaherpesvirus 3

Storage Stability

Storage Stability Organism
-70°C, stable for several months Human alphaherpesvirus 3

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + thymidine
-
 Human alphaherpesvirus 3 ADP + dTMP
-
 ?
ATP + thymidine
-
 Human alphaherpesvirus 3 ?
-
 ?

Subunits

Subunits Comment Organism
dimer homodimer Human alphaherpesvirus 3