Cloned (Comment) | Organism |
---|---|
recombinant coexpression of N-terminally His-tagged full-length wild-type and mutant class 1a isoform catalytic subunits with the p85 regulatory subunit, impacct of the His-tag on the catalytic activity, overview | Homo sapiens |
recombinant coexpression of N-terminally His-tagged full-length wild-type and mutant class 1b isoform catalytic subunits with the p85alpha regulatory subunit, impact of the His-tag on the catalytic activity, overview | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E545K | site-directed mutagenesis of p110alpha, an oncogenic mutant enzyme form | Homo sapiens |
H1047R | site-directed mutagenesis of p110alpha, an oncogenic mutant enzyme form | Homo sapiens |
additional information | N-terminal tags on the catalytic (p110) subunit of phosphoinositol 3-kinase increase cell signalling and oncogenic transformation | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
- |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Homo sapiens | - |
ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27986 AND P42336 | class Ia enzyme, subunits alpha and beta; class Ia enzyme | - |
Homo sapiens | P42338 AND O00329 | class Ib enzyme, subunits alpha and beta; class Ib enzyme | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
Homo sapiens | ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1* 85000, alpha-subunit, + 1 * 110000, beta-subunit | Homo sapiens |
More | the heterodimers consisting of an 85 kDa regulatory/adapter subunit (p85) coupled to a 110 kDa catalytic subunit (p110) with both subunits possessing several isoforms | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
phosphoinositol 3-kinase | - |
Homo sapiens |
PI 3-K | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | class 1 phosphoinositide 3-kinases are heterodimers consisting of an 85 kDa regulatory/adapter subunit (p85) coupled to a 110 kDa catalytic subunit (p110) with both subunits possessing several isoforms. The class I enzymes are further subdivided into two subclasses: class Ia and class Ib. The class 1a phosphoinositol 3-kinases (p110alpha, p110beta and p110delta) signal downstream of tyrosine kinases, while the single class Ib phosphoinositol 3-kinase (p110gamma) operates downstream of heterotrimeric GPCRs (G-protein-coupled receptors) | Homo sapiens |
additional information | N-terminal tags on the catalytic (p110) subunit of phosphoinositol 3-kinase increase cell signalling and oncogenic transformation, the increase in cell signalling and oncogenic-like transformation in response to p110 NTT is not mediated via an increase in the lipid kinase activity of the enzyme, but may be mediated by increased p110 autophosphorylation and/or other intracellular protein/protein interactions. Tagged recombinant protein is suitable for use in in vitro lipid kinase screens to identify phosphoinositol 3-kinase inhibitors, while in vivo (including intracellular) experiments and investigations into the protein kinase activity should be conducted with untagged constructs | Homo sapiens |