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Literature summary for 2.7.1.15 extracted from

  • Li, J.; Wang, C.; Wu, Y.; Wu, M.; Wang, L.; Wang, Y.; Zang, J.
    Crystal structure of Sa239 reveals the structural basis for the activation of ribokinase by monovalent cations (2012), J. Struct. Biol., 177, 578-582.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of Sa239 in the absence of monovalent ions. The structure of Sa239 demonstrates that the C-terminal tail protrudes from the remaining part and interacts with the neighboring molecule, resulting in an dimerization form Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.19
-
ATP pH 8.0, 30°C, 100 mM K+ Staphylococcus aureus
4.66
-
ATP pH 8.0, 30°C, 0 mM K+ Staphylococcus aureus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ monovalent ions such as K+ are able to enhance substrate affinity of Sa239 and activate the reaction catalyzed by Sa239 Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus A0A0H2WZY4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-ribose
-
Staphylococcus aureus ADP + D-ribose 5-phosphate
-
?

Subunits

Subunits Comment Organism
dimer crystal structure Staphylococcus aureus

Synonyms

Synonyms Comment Organism
Sa239
-
Staphylococcus aureus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20.65
-
ATP pH 8.0, 30°C, 0 mM K+ Staphylococcus aureus
47.81
-
ATP pH 8.0, 30°C, 100 mM K+ Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Staphylococcus aureus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.42
-
ATP pH 8.0, 30°C, 0 mM K+ Staphylococcus aureus
21.87
-
ATP pH 8.0, 30°C, 100 mM K+ Staphylococcus aureus