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Literature summary for 2.7.1.15 extracted from
- Physical and functional interaction between D-ribokinase and topoisomerase I has opposite effects on their respective activity in Mycobacterium smegmatis and Mycobacterium tuberculosis (2011), Arch. Biochem. Biophys., 512, 135-142.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| MtbTopA | when increasing amounts of Mycobacterium smegmatis topoisomerase I are mixed together with ribokinase, the amount of phosphorylated D-ribose products steadily increases, indicating that Mycobacterium smegmatis topoisomerase I stimulates the kinase activity of ribokinase | Mycobacterium tuberculosis | |
| topoisomerase I | when increasing amounts of MsTopA are mixed together with ribokinase, the amount of phosphorylated D-ribose products steadily increases, indicating that topoisomerase I stimulates the kinase activity of Ms4585 | Mycolicibacterium smegmatis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Mycolicibacterium smegmatis |
| expressed in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A13/103/116V | mutant shows no interaction with topoisomerase I. Mutant only partially retains its ability to inhibit the activity of topoisomerase I even at high protein concentrations. Topoisomerase I does not significantly stimulate the activity of the mutant protein | Mycolicibacterium smegmatis |
| S11N/A14V | mutant shows no interaction with topoisomerase I | Mycobacterium tuberculosis |
| S11N/A14V | mutant shows no interaction with topoisomerase I. Mutant only partially retains its ability to inhibit the activity of topoisomerase I even at high protein concentrations. Topoisomerase I does not significantly stimulate the activity of the mutant protein | Mycolicibacterium smegmatis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Mycolicibacterium smegmatis | mycobacterial topoisomerase I (TopA) is found to physically interact with ribokinase both in vitro and in vivo. The interaction between ribokinase and TopA has opposite effects on their respective function. While the interaction between the two proteins inhibits the ability of TopA to relax supercoiled DNA, it stimulates ribokinase activity | ? | - |
? |  |
| additional information | Mycobacterium tuberculosis | mycobacterial topoisomerase I (TopA) is found to physically interact with ribokinase both in vitro and in vivo. The interaction between ribokinase and topoisomerase I has opposite effects on their respective function. While the interaction between the two proteins inhibits the ability of topoisomerase I to relax supercoiled DNA, it stimulates ribokinase activity | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycolicibacterium smegmatis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-ribose | - |
Mycolicibacterium smegmatis | ADP + D-ribose 5-phosphate | - |
? | |
| ATP + D-ribose | - |
Mycobacterium tuberculosis | ADP + D-ribose 5-phosphate | - |
? | |
| additional information | mycobacterial topoisomerase I (TopA) is found to physically interact with ribokinase both in vitro and in vivo. The interaction between ribokinase and TopA has opposite effects on their respective function. While the interaction between the two proteins inhibits the ability of TopA to relax supercoiled DNA, it stimulates ribokinase activity | Mycolicibacterium smegmatis | ? | - |
? | |
| additional information | mycobacterial topoisomerase I (TopA) is found to physically interact with ribokinase both in vitro and in vivo. The interaction between ribokinase and topoisomerase I has opposite effects on their respective function. While the interaction between the two proteins inhibits the ability of topoisomerase I to relax supercoiled DNA, it stimulates ribokinase activity | Mycobacterium tuberculosis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-ribokinase | - |
Mycolicibacterium smegmatis |
| D-ribokinase | - |
Mycobacterium tuberculosis |
| Ms4585 | - |
Mycolicibacterium smegmatis |
| Rv2436 | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mycolicibacterium smegmatis |
| 37 | - |
assay at | Mycobacterium tuberculosis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | ribokinase inhibits the function of MsTopA in relaxing supercoiled DNA | Mycolicibacterium smegmatis |
| physiological function | ribokinase inhibits the function of topoisomerase I in relaxing supercoiled DNA | Mycobacterium tuberculosis |
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