Cloned (Comment) | Organism |
---|---|
stable expression of GFP-tagged Atg14 in NIH3T3 cells, transient co-expression of N-terminally Myc-, HA-, or FLAG-tagged Atg14, Beclin 1, and UVRAG in HEK-293T cells | Homo sapiens |
transient co-expression of N-terminally Myc-, HA-, or FLAG-tagged Beclin 1 in HEK-293T cells | Homo sapiens |
transient co-expression of N-terminally Myc-, HA-, or FLAG-tagged UVRAG in HEK-293T cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | silencing by siRNA-mediated knockdown of Atg14 in HeLa cells suppresses autophagosome formation | Homo sapiens |
additional information | silencing by siRNA-mediated knockdown of UVRAG in HeLa cells suppresses autophagosome formation | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endosome | - |
Homo sapiens | 5768 | - |
membrane | - |
Homo sapiens | 16020 | - |
additional information | Atg14 is present on autophagic isolation membranes | Homo sapiens | - |
- |
additional information | UVRAG primarily associates with Rab9-positive endosomes | Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | Vps34, p150, Beclin 1, Atg14, and UVRAG form two distinct class III phosphatidylinositol 3-kinase complexes, overview. Atg14 interacts with Beclin 1 and Vps34 but not with UVRAG, the coiled-coil region of Atg14 required for binding with Vps34 and Beclin 1 is essential for autophagy | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
Vps34, p150, Beclin 1, Atg14, and UVRAG form two distinct class III phosphatidylinositol 3-kinase complexes | - |
Homo sapiens | Q6ZNE5 | Atg14; Vps34, p150, Beclin 1, Atg14, and UVRAG form two distinct class III phosphatidylinositol 3-kinase complexes | - |
Homo sapiens | Q9P2Y5 | UVRAG; Vps34, p150, Beclin 1, Atg14, and UVRAG form two distinct class III phosphatidylinositol 3-kinase complexes | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Vps34, p150, Beclin 1, Atg14, and UVRAG form two distinct class III phosphatidylinositol 3-kinase complexes, overview. Atg14 interacts with Beclin 1 and Vps34 but not with UVRAG, the coiled-coil region of Atg14 required for binding with Vps34 and Beclin 1 is essential for autophagy | Homo sapiens | ? | - |
? | |
additional information | PI3-kinase activity is not required for starvation-induced Atg14 puncta formation | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | Vps34 contains three distinct domains: the N-terminal C2 domain, central accessory domain and C-terminal catalytic domain | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
class III phosphatidylinositol 3-kinase | - |
Homo sapiens |
PI3-kinase | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | mammalian cells have at least two distinct class III PI3-kinase complexes, which may function in different membrane trafficking pathways | Homo sapiens |