Literature summary for 2.7.1.137 extracted from

Meier, T.I.; Cook, J.A.; Thomas, J.E.; Radding, J.A.; Horn, C.; Lingaraj, T.; Smith, M.C.
Cloning, expression, purification, and characterization of the human Class Ia phosphoinositide 3-kinase isoforms (2004), Protein Expr. Purif., 35, 218-224.

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Cloned(Commentary)

Cloned (Comment)	Organism
class Ia isozymes, DNA sequence determination and analysis, expression of His-tagged isozymes or P85 and p110 subunits alone in Spodoptera frugiperda Sf9 insect cells utilizing the baculovirus infection system	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
85000	-	1 * 128900, catalytic subunit, recombinant isozyme alpha, + 1 * 83400, regulatory subunit, recombinant isozyme alpha, mass spectrometry, 1 * 110000, catalytic subunit, + 1 * 85000, regulatory subunit, recombinant isozymes alpha-delta, SDS-PAGE	Homo sapiens
110000	-	1 * 128900, catalytic subunit, recombinant isozyme alpha, + 1 * 83400, regulatory subunit, recombinant isozyme alpha, mass spectrometry, 1 * 110000, catalytic subunit, + 1 * 85000, regulatory subunit, recombinant isozymes alpha-delta, SDS-PAGE	Homo sapiens
128900	-	1 * 128900, catalytic subunit, recombinant isozyme alpha, + 1 * 83400, regulatory subunit, recombinant isozyme alpha, mass spectrometry, 1 * 110000, catalytic subunit, + 1 * 85000, regulatory subunit, recombinant isozymes alpha-delta, SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + phosphatidylinositol-4,5-bisphosphate	Homo sapiens	class Ia isozyme	ADP + phosphatidylinositol-3,4,5-trisphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	class Ia phosphoinositide 3-kinase isozymes alpha, beta, gamma, and delta	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged class Ia isozymes and recombinant His-tagged p85 and p110 subunits from insect cells by nickel affinity chromatography to homogeneity	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	optimization of substrate surface concentration in enzyme assays for each isozyme, overview	Homo sapiens
0.0016	-	purified recombinant isozyme gamma, at phosphatidylinositol-4,5-bisphosphate surface concentration of 10 mol%	Homo sapiens
0.0017	-	purified recombinant isozyme beta, at phosphatidylinositol-4,5-bisphosphate surface concentration of 7.5 mol%	Homo sapiens
0.0028	-	purified recombinant isozyme delta, at phosphatidylinositol-4,5-bisphosphate surface concentration of 2.5 mol%	Homo sapiens
0.0086	-	purified recombinant isozyme alpha, at phosphatidylinositol-4,5-bisphosphate surface concentration of 10 mol%	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + phosphatidylinositol-4,5-bisphosphate	class Ia isozyme	Homo sapiens	ADP + phosphatidylinositol-3,4,5-trisphosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	1 * 128900, catalytic subunit, recombinant isozyme alpha, + 1 * 83400, regulatory subunit, recombinant isozyme alpha, mass spectrometry, 1 * 110000, catalytic subunit, + 1 * 85000, regulatory subunit, recombinant isozymes alpha-delta, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
phosphoinositide 3-kinase	-	Homo sapiens
PI3K	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at room temperature	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

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Release 2023.1 (January 2023)