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Literature summary for 2.7.1.11 extracted from

  • Kloos, M.; Brueser, A.; Kirchberger, J.; Schoeneberg, T.; Straeter, N.
    Crystal structure of human platelet phosphofructokinase-1 locked in an activated conformation (2015), Biochem. J., 469, 421-432.
    View publication on PubMed
Search for more literature for 2.7.1.11

Activating Compound

Activating Compound Comment Organism Structure
fructose 6-phosphate S0.5 value for wild-type 0.88 mM, in presence of 1 mM AMP S0.5 value 0.052 mM. S0.5 value for N-terminal truncation mutant 0.089 mM, in presence of 1 mM AMP S0.5 value 0.030 mM Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
trunctation mutant in complex with ATP and with ADP, fructose 6-phosphate and fructose 1,6-bisphosphate, to 2.67-2.80 A resolution Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of a trunctation mutant lacking the first N-terminal 24 amino acids. The inhibiting effect of citrate is abolished in the mutant, and the truncated variant is permanently activated even in the absence of AMP Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 ATP N-terminal truncation mutant, pH 7.1, 25°C Homo sapiens
0.037
-
 ATP wild-type, pH 7.1, 25°C Homo sapiens
0.076
-
 ATP N-terminal truncation mutant, presence of 5 mM AMP, pH 7.1, 25°C Homo sapiens
0.16
-
 ATP wild-type, presence of 5 mM AMP, pH 7.1, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q01813 platelet-type isoform
-

Source Tissue

Source Tissue Comment Organism Textmining
blood platelet
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Homo sapiens ADP + D-fructose 1,6-bisphosphate
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6
-
 ATP wild-type, pH 7.1, 25°C Homo sapiens
4.6
-
 ATP N-terminal truncation mutant, pH 7.1, 25°C Homo sapiens