Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fructose 6-phosphate | S0.5 value for wild-type 0.88 mM, in presence of 1 mM AMP S0.5 value 0.052 mM. S0.5 value for N-terminal truncation mutant 0.089 mM, in presence of 1 mM AMP S0.5 value 0.030 mM | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
trunctation mutant in complex with ATP and with ADP, fructose 6-phosphate and fructose 1,6-bisphosphate, to 2.67-2.80 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a trunctation mutant lacking the first N-terminal 24 amino acids. The inhibiting effect of citrate is abolished in the mutant, and the truncated variant is permanently activated even in the absence of AMP | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
ATP | N-terminal truncation mutant, pH 7.1, 25°C | Homo sapiens | |
0.037 | - |
ATP | wild-type, pH 7.1, 25°C | Homo sapiens | |
0.076 | - |
ATP | N-terminal truncation mutant, presence of 5 mM AMP, pH 7.1, 25°C | Homo sapiens | |
0.16 | - |
ATP | wild-type, presence of 5 mM AMP, pH 7.1, 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q01813 | platelet-type isoform | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood platelet | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Homo sapiens | ADP + D-fructose 1,6-bisphosphate | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
ATP | wild-type, pH 7.1, 25°C | Homo sapiens | |
4.6 | - |
ATP | N-terminal truncation mutant, pH 7.1, 25°C | Homo sapiens |