Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Mycobacterium tuberculosis | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Mycobacterium tuberculosis H37Rv | ADP + D-fructose 1,6-bisphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PFKA | isozyme | Mycobacterium tuberculosis |
phosphofructokinase | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
physiological function | isozyme PFKA is required for growth on glucose as sole carbon source. The isoform is also required for survival of hypoxic non-replicating Mycobaterium tuberculosis but is not required for virulence and survival in the mouse model of tuberculosis infection | Mycobacterium tuberculosis |