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Literature summary for 2.7.1.11 extracted from

  • Banaszak, K.; Mechin, I.; Obmolova, G.; Oldham, M.; Chang, S.H.; Ruiz, T.; Radermacher, M.; Kopperschlaeger, G.; Rypniewski, W.
    The crystal structures of eukaryotic phosphofructokinases from bakers yeast and rabbit skeletal muscle (2011), J. Mol. Biol., 407, 284-297.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 2,6-bisphosphate
-
Oryctolagus cuniculus
D-fructose 2,6-bisphosphate
-
Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expressed in a pfk-deficient Escherichia coli strain DF1020DE3 Oryctolagus cuniculus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 16% (w/v) PEG 400, 0.1 M MgSO4, and 0.1 M acetate buffer (pH 5.3) Oryctolagus cuniculus
hanging drop vapor diffusion method, using 6-10% (w/v) PEG 4000 and 0.2 mM sodium acetate in 0.1 M 4-morpholineethanesulfonic acid buffer (pH 6.0) Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus P00511
-
-
Saccharomyces cerevisiae P16862
-
-

Purification (Commentary)

Purification (Comment) Organism
Cibacron Blue 3GA gel filtration, DE-52 column chromatography and ATP-N6-agarose column chromatography Oryctolagus cuniculus
PEG 6000 precipitation at 3.5% and 14% (w/v) and Cibacron Blue F3G-A Sephadex G 100 gel filtration Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
Oryctolagus cuniculus ADP + D-fructose 1,6-bisphosphate
-
?
ATP + D-fructose 6-phosphate
-
Saccharomyces cerevisiae ADP + D-fructose 1,6-bisphosphate
-
?

Subunits

Subunits Comment Organism
heterooctamer x-ray crystallography Saccharomyces cerevisiae
heterotetramer x-ray crystallography Oryctolagus cuniculus

Synonyms

Synonyms Comment Organism
PFK
-
Oryctolagus cuniculus
PFK
-
Saccharomyces cerevisiae
phosphofructokinase 1
-
Oryctolagus cuniculus
phosphofructokinase 1
-
Saccharomyces cerevisiae